Detailed information of TA system TA188856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 6864..7357
• Replicon: plasmid p33Kpn22-KPC
• Accession: NZ_CP069050
• Organism: Klebsiella pneumoniae strain 33Kpn22

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: IFY68_RS29795
• Protein ID: WP_001230709.1
• Coordinates: 6864..7118 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: IFY68_RS29800
• Protein ID: WP_000151456.1
• Coordinates: 7115..7357 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFY68_RS29760	2438..2599	+	162	WP_001434039.1	type I toxin-antitoxin system Hok family toxin	-
IFY68_RS29765	2749..3345	+	597	WP_000125164.1	hypothetical protein	-
IFY68_RS29770	3363..3647	-	285	WP_001162371.1	hypothetical protein	-
IFY68_RS29775	3721..4386	-	666	WP_000532148.1	P-loop NTPase	-
IFY68_RS29780	4973..5302	+	330	WP_001542022.1	hypothetical protein	-
IFY68_RS29785	5706..6221	+	516	WP_001025393.1	J domain-containing protein	-
IFY68_RS29790	6325..6867	+	543	WP_001083904.1	hypothetical protein	-
IFY68_RS29795	6864..7118	+	255	WP_001230709.1	hypothetical protein	Toxin
IFY68_RS29800	7115..7357	+	243	WP_000151456.1	hypothetical protein	Antitoxin
IFY68_RS29805	7938..8261	+	324	WP_020314677.1	hypothetical protein	-
IFY68_RS29810	8303..8512	+	210	WP_020314678.1	hypothetical protein	-
IFY68_RS29815	8609..8833	+	225	WP_000814381.1	hypothetical protein	-
IFY68_RS29820	8885..9145	+	261	WP_020314676.1	hypothetical protein	-
IFY68_RS29825	9135..9374	+	240	WP_022540736.1	transcriptional regulator	-
IFY68_RS29830	9909..10169	+	261	WP_020314977.1	hypothetical protein	-
IFY68_RS29835	10556..10738	-	183	WP_001401700.1	hypothetical protein	-
IFY68_RS29840	11283..11465	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
IFY68_RS29845	11490..11927	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-3 / blaTEM-1A	-	1..74767	74767

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9942.47 Da
• Isoelectric Point: 10.1206

>T188856 WP_001230709.1 NZ_CP069050:6864-7118 [Klebsiella pneumoniae]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Nucleotide

• Length: 255 bp

>T188856 NZ_CP090588:c3166104-3165700 [Pectobacterium aroidearum]
ATGCTGAAATATCTACTTGATACCAATATCTGTATTTACACCATTAAGAACAAACCGCAGGAAGTCAGGGACGCTTTCTA
CCGCCATTACGGGCAGTTTGCTATCAGTTCTATCACACTGATGGAACTAATCGATGGTGCGGAGAAATCGGTAAACCCAG
AAAAGAATCTCGCCGTGATTGAAGGTTTTTCTGCTCGTCTGGAAGTGCAAACGTATGGCTTTGACGCTGCTGTACATACC
GGGCAAATTCGTGCCGAGCTGGCAAGACAAGGAACCCCTATCGGCCCGTATGATGCCATGTTAGCCGGTCATGCTCGGTC
AGCAGGTTTGATACTGGTAACGAACAATGTGCGTGAGTTTGAGCGTGTGCCAGGATTGCGCGTAGAAAACTGGGTCGGTA
GATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT188856 WP_000151456.1 NZ_CP069050:7115-7357 [Klebsiella pneumoniae]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT188856 NZ_CP090588:c3166334-3166104 [Pectobacterium aroidearum]
ATGGAAAAAACCACAGTATTTAAAAGCAATCGCAGCCAGGCGGTACGGCTCCCTAAAGCCGTTGCACTGCCCGATGATGT
GAAGCAGGTTGATATCGTTGCGATTGGTCGAACACGTATCATTACTCCGGCAGGCGAAAGCTGGGACAGTTGGTTCGAAG
AGGACGGGGTAACGCCTGACTTTATGATCACCAGAGAGCAACCCGATGATCAGATCAGGGAAGATTTTTGA

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5