Detailed information of TA system TA188854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 149470..150047
• Replicon: plasmid p33Kpn22-2
• Accession: NZ_CP069048
• Organism: Klebsiella pneumoniae strain 33Kpn22

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IFY68_RS28890
• Protein ID: WP_023286013.1
• Coordinates: 149715..150047 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: IFY68_RS28885
• Protein ID: WP_098992514.1
• Coordinates: 149470..149715 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFY68_RS28845	144597..145046	+	450	WP_203162963.1	hypothetical protein	-
IFY68_RS28850	145103..145333	+	231	WP_045268436.1	hypothetical protein	-
IFY68_RS28855	145451..145888	+	438	WP_203162964.1	plasmid protein	-
IFY68_RS28860	145885..146571	+	687	WP_196558489.1	hypothetical protein	-
IFY68_RS28865	146595..147212	+	618	WP_203162965.1	hypothetical protein	-
IFY68_RS28870	147740..148414	+	675	WP_098992517.1	hypothetical protein	-
IFY68_RS28875	148494..148766	+	273	WP_098992516.1	hypothetical protein	-
IFY68_RS28880	148832..149389	+	558	WP_098992515.1	hypothetical protein	-
IFY68_RS28885	149470..149715	+	246	WP_098992514.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IFY68_RS28890	149715..150047	+	333	WP_023286013.1	endoribonuclease MazF	Toxin
IFY68_RS28895	150322..150642	+	321	WP_023286012.1	hypothetical protein	-
IFY68_RS28900	150768..151406	+	639	WP_052684048.1	hypothetical protein	-
IFY68_RS28905	151418..151720	+	303	WP_023286010.1	hypothetical protein	-
IFY68_RS28910	151812..152231	+	420	WP_098992512.1	hypothetical protein	-
IFY68_RS28915	152281..152595	+	315	WP_023286008.1	hypothetical protein	-
IFY68_RS28920	152585..152791	+	207	WP_098992511.1	hypothetical protein	-
IFY68_RS28925	152960..153820	+	861	WP_203162966.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..174178	174178

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• Antitoxin PemI-like (5-80)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11943.82 Da
• Isoelectric Point: 8.7208

>T188854 WP_023286013.1 NZ_CP069048:149715-150047 [Klebsiella pneumoniae]
MVKRYVPDAGDLIWIDFDPVAGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTQVKGYPFEVTLSGSKEGVALSDQITCV
DWRARKVTQKSTVSKSELAEIRAKAKALIG

Nucleotide

• Length: 333 bp

>T188854 NZ_CP090588:207775-208050 [Pectobacterium aroidearum]
ATGCAGGGAAAAATAGCCAGTCTACGAAAAAAGCAGCGAGAAACGCTGAGCCAAATTTTCAAAACACCCGTGCTTTCAGG
TGTTAAATGGTCAAATGTTGAGTCACTCATCACTGCGTTAGGTGGTGAAATTAAGGAAGGTAGCGGGTCACGGGTGCGAT
TTTTATTGAATGGGAGTATCGCCCGGTTCCATCGTCCTCACCCGTCACCGGATACTGATAAAGGCGCATTGGTTAACCTG
CGTGAATGGTTGGAAAGTATAGGAGTAAAACCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8700.03 Da
• Isoelectric Point: 5.0009

>AT188854 WP_098992514.1 NZ_CP069048:149470-149715 [Klebsiella pneumoniae]
MAQAIVKKWGNSPSVRLPVAVMKAASLNVDDTVDIDVEDGRIIIVPARPKEYSLDALLSGISSDNLHDKVDFGPAKGKEL
L

Nucleotide

• Length: 246 bp

>AT188854 NZ_CP090588:208047-208409 [Pectobacterium aroidearum]
ATGACTAAAGCACTTAACACACCAAATACGATGGTTGTTTCCGGCCAACCGGCCATTATCAGCTATGTACCGGAAATCGG
TATGTTTCGCGGTAAGTTTATTGGCTTATCTGGCTACTGTGATTTTGTCGCTGACAGTATTAATGGGCTGAGAAACGAGG
GCGAAATTTCGCTACGTGAATATCTGGAAGACTGTCAGGAAAATGGCATTGAACCTTACGAACGTGAAGAGAAGGTGAAA
ACGTTCACTCTCCGTTATCCTGAATCATTCGGAGAACGACTGACTATTGCGGCGGCTGAACGTCAAATTTCAGTGAATAC
GTTTATTGTCGAAACGCTTAATGAACGTATGAAACAGGCTTAA