Detailed information of TA system TA188853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 55201..55937
• Replicon: plasmid p33Kpn22-1
• Accession: NZ_CP069047
• Organism: Klebsiella pneumoniae strain 33Kpn22

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: IFY68_RS27220
• Protein ID: WP_003026803.1
• Coordinates: 55455..55937 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: IFY68_RS27215
• Protein ID: WP_003026799.1
• Coordinates: 55201..55467 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFY68_RS27170	51263..51625	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
IFY68_RS27175	51675..52025	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
IFY68_RS27180	52383..52652	+	270	WP_004152102.1	hypothetical protein	-
IFY68_RS27185	52640..53215	+	576	WP_004152103.1	hypothetical protein	-
IFY68_RS27190	53246..53740	+	495	WP_004152104.1	DNA-binding protein	-
IFY68_RS27195	53784..54152	+	369	WP_004152105.1	hypothetical protein	-
IFY68_RS27200	54186..54389	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
IFY68_RS27205	54438..54695	+	258	WP_004152107.1	hypothetical protein	-
IFY68_RS27210	54771..55025	+	255	WP_004152108.1	hypothetical protein	-
IFY68_RS27215	55201..55467	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
IFY68_RS27220	55455..55937	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
IFY68_RS27225	56149..57495	+	1347	WP_020314316.1	ISNCY family transposase	-
IFY68_RS27230	57996..59250	-	1255	Protein_59	IS3 family transposase	-
IFY68_RS27235	59338..60300	-	963	WP_004152113.1	M48 family metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA1	-	1..196778	196778

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T188853 WP_003026803.1 NZ_CP069047:55455-55937 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T188853 NZ_CP090588:c26412-26026 [Pectobacterium aroidearum]
ATGGAGGTTTACAGATTGGCATGGGAAATTGTCACACGGGCGCTATTCGATACGTGGTTTGATGAGCAAACCGAAACGGT
ACAGGAAGAGGTTTTGGCACACCTCGGTATACTCGAAGAAGATGGCCCACAGCTAGGACGACCACAGGTTGATCAGATTA
AAGGCTCACAATACAAAAATATGAAGGAGCTTCGAGTTCAGGTTGGCGGCCACCCATTCCGAACATTCTTCGCGTTTGAC
CGGACACGTAAAGCGATTGTTTTATGTGCGGGAGATAAGAAAGGCGAAAATGAAAAATTGTTCTACGAACGTATGATTAA
GATCGCAGACGCTGAGTTTACATCGCATCTGATATCACCGGAGGTAAAAGAAGATGGCGACATTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT188853 WP_003026799.1 NZ_CP069047:55201-55467 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT188853 NZ_CP090588:c26039-25740 [Pectobacterium aroidearum]
ATGGCGACATTTAAAGAAATGATGGCTAAACGTTCACCGGAGAGCCGCGCCCGCATTGAAGCGCGCACGGCTGAGATCCG
TCAGGAAATCACTTTAGCGAAACTACGCGAAGAGCTGAACATGTCGCAGACTCAGCTTGCATTAGCTCTGGGCGTCAAGC
AGCCATCTATTGTTAAGATGGAGAAGGTCGAGAATGACCCAAAACTGTCAACGCTGAAACGCTATGTCACTGCCCTAGGC
GGCAAGCTTACGCTAGATGTGACCTTGCCTGATGGTAAGCGTATCGCGCTTACTTTGTAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66