Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5423614..5424239 Replicon chromosome
Accession NZ_CP069046
Organism Klebsiella pneumoniae strain 33Kpn22

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IFY68_RS26570 Protein ID WP_002882817.1
Coordinates 5423614..5423997 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IFY68_RS26575 Protein ID WP_004150355.1
Coordinates 5423997..5424239 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFY68_RS26555 5420980..5421882 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IFY68_RS26560 5421879..5422514 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IFY68_RS26565 5422511..5423440 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IFY68_RS26570 5423614..5423997 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IFY68_RS26575 5423997..5424239 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IFY68_RS26580 5424444..5425361 + 918 WP_002882812.1 alpha/beta hydrolase -
IFY68_RS26585 5425375..5426316 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IFY68_RS26590 5426361..5426798 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IFY68_RS26595 5426795..5427655 - 861 WP_002882807.1 virulence factor BrkB family protein -
IFY68_RS26600 5427649..5428248 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T188852 WP_002882817.1 NZ_CP069046:c5423997-5423614 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T188852 NZ_CP090587:4877925-4878212 [Pectobacterium aroidearum]
ATGAGCTATAGCGTCAAATTCAGGGAAGATGCGCTGAAAGAATGGCTCAAGCTCGATAAAACCATCCAACAGCAATTTGC
CAAAAAACTAAAAAAATGCTGCGAAAATCCTCATATTCCTTCGGCAAAGTTACGGGGGATGAAGGACTGCTACAAAATAA
AGCTCCGAGCATCAGGCTTTCGTTTGGTTTATGAAGTCATTGACGACATCTTGATTATTGCCGTGGTGGCGGTAGGTAAA
CGTGAACGCAGCGGTGTTTATCATCTGGCGAGTGAGCGAATGAGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT188852 WP_004150355.1 NZ_CP069046:c5424239-5423997 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT188852 NZ_CP090587:4877677-4877928 [Pectobacterium aroidearum]
ATGCCGAATATTATATTGAGTGATACCAGTGCCAGCGTCAGTGAATTGAAAAAGAACCCGATGGCGACGGTCAATGCTGG
CGACGGTCTACCTGTCGCTATTTTGAACCGCAACCAGCCTGCGTTTTATTGCATTCCTGCGGATCTTTACGAAAAAATGC
TGGATGCGCTGGACGACCAGGAACTGGTTAAGCTAATCGGTGAACGCAAAAACCAGCCGCTGGTTGACGTGGATTTGGAT
AGTTTCCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References