Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4844223..4845033 Replicon chromosome
Accession NZ_CP069046
Organism Klebsiella pneumoniae strain 33Kpn22

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag IFY68_RS23805 Protein ID WP_002887280.1
Coordinates 4844223..4844756 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag IFY68_RS23810 Protein ID WP_002887278.1
Coordinates 4844767..4845033 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFY68_RS23800 4843054..4844175 + 1122 WP_002887282.1 cupin domain-containing protein -
IFY68_RS23805 4844223..4844756 - 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
IFY68_RS23810 4844767..4845033 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
IFY68_RS23815 4845136..4846569 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
IFY68_RS23820 4846559..4847242 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
IFY68_RS23825 4847414..4848799 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
IFY68_RS23830 4848817..4849161 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T188849 WP_002887280.1 NZ_CP069046:c4844756-4844223 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T188849 NZ_CP090587:4451822-4452076 [Pectobacterium aroidearum]
GTGAAGCTAATCTGGTCAGAAGAAGCATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGGATGGTCAAAAAAAT
CAATGAATTAATAAAAGAAACGCGCAGAACACCTTTTGAGGGAAAAGGAAAACCAGAGCCACTTAAACATAATCTTGCTG
GTTTCTGGTCGCGCCGTATCACCGAAGAACATCGTCTGGTTTATGCCATCACCGATGACGCCATGATGATCGCTGCCTGC
CGCTACCACTATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT188849 WP_002887278.1 NZ_CP069046:c4845033-4844767 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT188849 NZ_CP090587:4451574-4451825 [Pectobacterium aroidearum]
ATGCGTACAATTAGCTACAGTGAAGCCCGACAAAATCTATCGGCGACCATGATGAAAACGGTCGAAGACCGTGCTCCCAT
TCTTATCACTCGACAGAATGGTGAAGCCTGCGTGCTCATGTCTTTGGAAGAATATAACTCTCTAGAGGAGACCGCGTATT
TACTGCGTTCACCAGCAAATGCAAAAAGACTGATGAACTCAATCGAGAGCCTTAAAGCTGGCAATGGCGAAGAAAGAGAT
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References