Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4228919..4229538 Replicon chromosome
Accession NZ_CP069046
Organism Klebsiella pneumoniae strain 33Kpn22

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag IFY68_RS20840 Protein ID WP_002892050.1
Coordinates 4229320..4229538 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag IFY68_RS20835 Protein ID WP_002892066.1
Coordinates 4228919..4229293 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFY68_RS20825 4224071..4225264 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
IFY68_RS20830 4225287..4228433 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
IFY68_RS20835 4228919..4229293 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
IFY68_RS20840 4229320..4229538 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
IFY68_RS20845 4229697..4230263 + 567 WP_002892030.1 maltose O-acetyltransferase -
IFY68_RS20850 4230235..4230375 - 141 WP_004147370.1 hypothetical protein -
IFY68_RS20855 4230396..4230866 + 471 WP_002892026.1 YlaC family protein -
IFY68_RS20860 4230841..4232292 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
IFY68_RS20865 4232393..4233091 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
IFY68_RS20870 4233088..4233228 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
IFY68_RS20875 4233228..4233491 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T188848 WP_002892050.1 NZ_CP069046:4229320-4229538 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T188848 NZ_CP090587:4451574-4451825 [Pectobacterium aroidearum]
ATGCGTACAATTAGCTACAGTGAAGCCCGACAAAATCTATCGGCGACCATGATGAAAACGGTCGAAGACCGTGCTCCCAT
TCTTATCACTCGACAGAATGGTGAAGCCTGCGTGCTCATGTCTTTGGAAGAATATAACTCTCTAGAGGAGACCGCGTATT
TACTGCGTTCACCAGCAAATGCAAAAAGACTGATGAACTCAATCGAGAGCCTTAAAGCTGGCAATGGCGAAGAAAGAGAT
ATCATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT188848 WP_002892066.1 NZ_CP069046:4228919-4229293 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT188848 NZ_CP090587:4451822-4452076 [Pectobacterium aroidearum]
GTGAAGCTAATCTGGTCAGAAGAAGCATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGGATGGTCAAAAAAAT
CAATGAATTAATAAAAGAAACGCGCAGAACACCTTTTGAGGGAAAAGGAAAACCAGAGCCACTTAAACATAATCTTGCTG
GTTTCTGGTCGCGCCGTATCACCGAAGAACATCGTCTGGTTTATGCCATCACCGATGACGCCATGATGATCGCTGCCTGC
CGCTACCACTATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References