Detailed information of TA system TA188830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1240998..1241915
• Replicon: chromosome
• Accession: NZ_CP068989
• Organism: Bacillus velezensis strain WSM-1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: IPZ53_RS06435
• Protein ID: WP_007407256.1
• Coordinates: 1241169..1241915 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: IPZ53_RS06430
• Protein ID: WP_003154807.1
• Coordinates: 1240998..1241168 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPZ53_RS06395	1236231..1237853	+	1623	WP_032874601.1	pyocin knob domain-containing protein	-
IPZ53_RS06400	1237866..1238237	+	372	WP_032874603.1	YomQ/XkdW protein, phage-like element PBSX	-
IPZ53_RS06405	1238242..1238439	+	198	WP_032874605.1	XkdX family protein	-
IPZ53_RS06410	1238496..1239257	+	762	WP_032874607.1	hypothetical protein	-
IPZ53_RS06415	1239309..1239572	+	264	WP_003154815.1	hemolysin XhlA family protein	-
IPZ53_RS06420	1239586..1239849	+	264	WP_003154813.1	phage holin	-
IPZ53_RS06425	1239863..1240741	+	879	WP_032874609.1	N-acetylmuramoyl-L-alanine amidase	-
IPZ53_RS06430	1240998..1241168	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
IPZ53_RS06435	1241169..1241915	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
IPZ53_RS06440	1242020..1243018	-	999	WP_032874611.1	inorganic phosphate transporter	-
IPZ53_RS06445	1243031..1243648	-	618	WP_032874614.1	DUF47 domain-containing protein	-
IPZ53_RS06450	1243934..1245250	-	1317	WP_032874616.1	amino acid permease	-
IPZ53_RS06455	1245572..1246522	+	951	WP_032874618.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1194060..1299767	105707

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T188830 WP_007407256.1 NZ_CP068989:c1241915-1241169 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T188830 NZ_CP090574:6765-7013 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT188830 WP_003154807.1 NZ_CP068989:c1241168-1240998 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT188830 NZ_CP090574:7010-7357 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14