Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 489965..490602 Replicon chromosome
Accession NZ_CP068989
Organism Bacillus velezensis strain WSM-1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag IPZ53_RS02485 Protein ID WP_003156187.1
Coordinates 490252..490602 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag IPZ53_RS02480 Protein ID WP_003156188.1
Coordinates 489965..490246 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IPZ53_RS02460 486330..486929 - 600 WP_032872997.1 rhomboid family intramembrane serine protease -
IPZ53_RS02465 487022..487387 + 366 WP_007609580.1 holo-ACP synthase -
IPZ53_RS02470 487552..488559 + 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
IPZ53_RS02475 488676..489845 + 1170 WP_032873001.1 alanine racemase -
IPZ53_RS02480 489965..490246 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
IPZ53_RS02485 490252..490602 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
IPZ53_RS02490 490720..491541 + 822 WP_003156182.1 STAS domain-containing protein -
IPZ53_RS02495 491546..491911 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
IPZ53_RS02500 491914..492315 + 402 WP_003156178.1 anti-sigma regulatory factor -
IPZ53_RS02505 492327..493334 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
IPZ53_RS02510 493398..493727 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
IPZ53_RS02515 493724..494206 + 483 WP_007609591.1 anti-sigma B factor RsbW -
IPZ53_RS02520 494172..494960 + 789 WP_032873003.1 RNA polymerase sigma factor SigB -
IPZ53_RS02525 494960..495562 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T188829 WP_003156187.1 NZ_CP068989:490252-490602 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T188829 NZ_CP090574:4801-5052 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT188829 WP_003156188.1 NZ_CP068989:489965-490246 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT188829 NZ_CP090574:5039-5266 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References