Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 608166..608802 Replicon chromosome
Accession NZ_CP068988
Organism Bacillus paralicheniformis strain SUBG0010

Toxin (Protein)


Gene name mazF Uniprot ID M5P3Q9
Locus tag DI291_RS03045 Protein ID WP_003179128.1
Coordinates 608452..608802 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M5PDU2
Locus tag DI291_RS03040 Protein ID WP_006638778.1
Coordinates 608166..608447 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DI291_RS03020 603277..604761 + 1485 WP_103750290.1 PH domain-containing protein -
DI291_RS03025 604758..605357 - 600 WP_023857867.1 rhomboid family intramembrane serine protease -
DI291_RS03030 605701..606660 + 960 WP_191456887.1 DUF4367 domain-containing protein -
DI291_RS03035 606885..608054 + 1170 WP_191456885.1 alanine racemase -
DI291_RS03040 608166..608447 + 282 WP_006638778.1 hypothetical protein Antitoxin
DI291_RS03045 608452..608802 + 351 WP_003179128.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
DI291_RS03050 608920..609747 + 828 WP_023857863.1 RsbT co-antagonist protein RsbRA -
DI291_RS03055 609751..610116 + 366 WP_031305225.1 STAS domain-containing protein -
DI291_RS03060 610119..610520 + 402 WP_023857861.1 anti-sigma regulatory factor -
DI291_RS03065 610531..611538 + 1008 WP_020450261.1 PP2C family protein-serine/threonine phosphatase -
DI291_RS03070 611597..611923 + 327 WP_020450262.1 anti-sigma factor antagonist -
DI291_RS03075 611923..612405 + 483 WP_020450263.1 anti-sigma B factor RsbW -
DI291_RS03080 612371..613162 + 792 WP_023857858.1 RNA polymerase sigma factor SigB -
DI291_RS03085 613159..613758 + 600 WP_023857857.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12990.03 Da        Isoelectric Point: 5.7234

>T188827 WP_003179128.1 NZ_CP068988:608452-608802 [Bacillus paralicheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Download         Length: 351 bp

>T188827 NZ_CP090571:84760-85176 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10649.03 Da        Isoelectric Point: 4.6920

>AT188827 WP_006638778.1 NZ_CP068988:608166-608447 [Bacillus paralicheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Download         Length: 282 bp

>AT188827 NZ_CP090571:84533-84763 [Escherichia coli]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I7FHI4

Antitoxin

Source ID Structure
AlphaFold DB M5PDU2

References