Detailed information of TA system TA188821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4594512..4595371
• Replicon: chromosome
• Accession: NZ_CP068983
• Organism: Paradevosia shaoguanensis strain J5-3

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: JNE37_RS22345
• Protein ID: WP_174835044.1
• Coordinates: 4594512..4595042 (-)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: JNE37_RS22350
• Protein ID: WP_174838773.1
• Coordinates: 4595030..4595371 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNE37_RS22320	4590009..4590743	-	735	WP_203064878.1	SOS response-associated peptidase	-
JNE37_RS22325	4590831..4591406	-	576	WP_203064879.1	TetR/AcrR family transcriptional regulator	-
JNE37_RS22330	4591475..4591888	+	414	WP_203064880.1	VOC family protein	-
JNE37_RS22335	4591892..4592854	-	963	WP_203064881.1	50S ribosomal protein L3 N(5)-glutamine methyltransferase	-
JNE37_RS22340	4592983..4594158	+	1176	WP_203064882.1	ParB/RepB/Spo0J family partition protein	-
JNE37_RS22345	4594512..4595042	-	531	WP_174835044.1	GNAT family N-acetyltransferase	Toxin
JNE37_RS22350	4595030..4595371	-	342	WP_174838773.1	DUF1778 domain-containing protein	Antitoxin
JNE37_RS22355	4595823..4598117	+	2295	WP_203064883.1	AAA family ATPase	-
JNE37_RS22360	4598236..4599498	+	1263	WP_174835046.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	tet(G)	-	4594512..4615768	21256

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (50-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (24-112)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 19182.97 Da
• Isoelectric Point: 6.0974

>T188821 WP_174835044.1 NZ_CP068983:c4595042-4594512 [Paradevosia shaoguanensis]
MAEIDDTPDHPELRLSAPIPLTTEHDLSAFDCGEPVLNDWLRHRALKNESRFSRTYVVCAGNRVVGYFCISAGSVERSAA
PGKMRRNAPDLIPVSIIGRLAVSLDHAGKGLGMDLLSDALRRIAVASQSIGIGAVLVQAKDEAAKRFYLRCAEFIEYPED
SRTLFLPIETVVAGFG

Nucleotide

• Length: 531 bp

>T188821 NZ_CP090570:c2534885-2534709 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12371.14 Da
• Isoelectric Point: 10.7841

>AT188821 WP_174838773.1 NZ_CP068983:c4595371-4595030 [Paradevosia shaoguanensis]
MAKTRLAPSESSRSATAAVAVRASATDTKGSINLRIETGTRQLIDDAAAVLGKTRTEFMVESARRQAVDVLLDQRLFTLD
SERYDAFMQALDNPPAPGPKLKALLRRTPAWQK

Nucleotide

• Length: 342 bp

>AT188821 NZ_CP090570:c2534663-2534247 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA