Detailed information of TA system TA188818

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 2471361..2471926
• Replicon: chromosome
• Accession: NZ_CP068983
• Organism: Paradevosia shaoguanensis strain J5-3

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: JNE37_RS11840
• Protein ID: WP_035093697.1
• Coordinates: 2471678..2471926 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: JNE37_RS11835
• Protein ID: WP_203062931.1
• Coordinates: 2471361..2471681 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNE37_RS11800	2466401..2466853	-	453	WP_182399343.1	Hsp20 family protein	-
JNE37_RS11805	2467228..2468196	+	969	WP_203062927.1	alpha/beta hydrolase	-
JNE37_RS11810	2468471..2469295	-	825	WP_203062929.1	histidinol-phosphatase	-
JNE37_RS11815	2469564..2470472	-	909	WP_203062930.1	N-formylglutamate amidohydrolase	-
JNE37_RS11820	2470622..2470981	+	360	WP_035035553.1	response regulator	-
JNE37_RS11835	2471361..2471681	-	321	WP_203062931.1	BrnA antitoxin family protein	Antitoxin
JNE37_RS11840	2471678..2471926	-	249	WP_035093697.1	BrnT family toxin	Toxin
JNE37_RS11845	2472024..2472974	-	951	WP_203062933.1	cation transporter	-
JNE37_RS11850	2473176..2473649	+	474	WP_203062936.1	Cys-tRNA(Pro) deacylase	-
JNE37_RS11855	2474266..2476716	-	2451	WP_203062937.1	ATP-dependent helicase HrpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-101)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 8998.64 Da
• Isoelectric Point: 9.9246

>T188818 WP_035093697.1 NZ_CP068983:c2471926-2471678 [Paradevosia shaoguanensis]
MLISYDEAKRLTNLAKHGLDFEALTVEFFEAAVIFPAKVGRALAIGELNGQIVIAVVFRPLGSEALSVISMRPASRKERK
LL

Nucleotide

• Length: 249 bp

>T188818 NZ_CP090570:1020437-1020772 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11598.15 Da
• Isoelectric Point: 8.6568

>AT188818 WP_203062931.1 NZ_CP068983:c2471681-2471361 [Paradevosia shaoguanensis]
MTKKFASGRPLTDAEEAEIQRMIASDPDNPELTDEQIAAGGKPFAEVFPELAESIKRSRGRPKLSNAKQAVTLRLDPSTL
AKFQAAGDDWRTRMAEVLDKAKVGGR

Nucleotide

• Length: 321 bp

>AT188818 NZ_CP090570:1020189-1020437 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA