Detailed information of TA system TA188817

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2375584..2376365
• Replicon: chromosome
• Accession: NZ_CP068983
• Organism: Paradevosia shaoguanensis strain J5-3

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: JNE37_RS11370
• Protein ID: WP_203062814.1
• Coordinates: 2375871..2376365 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: JNE37_RS11365
• Protein ID: WP_203062813.1
• Coordinates: 2375584..2375874 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNE37_RS11345	2370785..2373754	+	2970	WP_203066375.1	Ti-type conjugative transfer relaxase TraA	-
JNE37_RS11350	2373839..2374609	-	771	WP_203066377.1	OST-HTH/LOTUS domain-containing protein	-
JNE37_RS11355	2375080..2375295	+	216	WP_203062811.1	AlpA family phage regulatory protein	-
JNE37_RS11360	2375285..2375518	+	234	WP_203062812.1	hypothetical protein	-
JNE37_RS11365	2375584..2375874	+	291	WP_203062813.1	DUF1778 domain-containing protein	Antitoxin
JNE37_RS11370	2375871..2376365	+	495	WP_203062814.1	GNAT family N-acetyltransferase	Toxin
JNE37_RS11375	2376383..2378077	+	1695	WP_203062815.1	recombinase family protein	-
JNE37_RS11380	2378443..2379435	-	993	WP_203062816.1	dipeptide ABC transporter ATP-binding protein	-
JNE37_RS11385	2379432..2380442	-	1011	WP_035094835.1	ABC transporter ATP-binding protein	-
JNE37_RS11390	2380452..2381168	-	717	WP_035094838.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2370785..2378077	7292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (39-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-85)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18274.04 Da
• Isoelectric Point: 8.0918

>T188817 WP_203062814.1 NZ_CP068983:2375871-2376365 [Paradevosia shaoguanensis]
VTGLRIEKLHRRHAVENFDCGEDALNRFLVRFALPNQMANASQTYVGVADDDAIVGFYTLVVGEVRYEEAPERLTKGLAR
HPVPVMLLARLGVSEAWQGKRIGAGLLRDAVLRTLQVADIAGVRALVVHAKNDAARAFYERFDFQPSPTDPLHLFALIKD
LRNL

Nucleotide

• Length: 495 bp

>T188817 NZ_CP090570:922622-923029 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10825.31 Da
• Isoelectric Point: 8.8517

>AT188817 WP_203062813.1 NZ_CP068983:2375584-2375874 [Paradevosia shaoguanensis]
MNTRITRTEKLDLRLTAEAKHTLAAAAQAQRRSLSEFVLESALGRAEEALADRRVFQLPPEKWEAFVAALDAPPRDLPRL
RKLLNEPGVFSDDNRS

Nucleotide

• Length: 291 bp

>AT188817 NZ_CP090570:922375-922641 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA