Detailed information of TA system TA188816

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 1957033..1957648
• Replicon: chromosome
• Accession: NZ_CP068983
• Organism: Paradevosia shaoguanensis strain J5-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JNE37_RS09260
• Protein ID: WP_203066041.1
• Coordinates: 1957033..1957428 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JNE37_RS09265
• Protein ID: WP_203066042.1
• Coordinates: 1957418..1957648 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNE37_RS09225	1952074..1952808	-	735	WP_203066036.1	uroporphyrinogen-III synthase	-
JNE37_RS09230	1952805..1953752	-	948	WP_203066037.1	hydroxymethylbilane synthase	-
JNE37_RS09235	1953785..1954885	+	1101	WP_203066038.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD	-
JNE37_RS09240	1954875..1955843	+	969	WP_203066039.1	NAD(P)-dependent glycerol-3-phosphate dehydrogenase	-
JNE37_RS09245	1955847..1956140	+	294	WP_203066040.1	YciI family protein	-
JNE37_RS09250	1956142..1956561	+	420	WP_035037291.1	EVE domain-containing protein	-
JNE37_RS09255	1956632..1957036	+	405	WP_182399030.1	DUF1761 domain-containing protein	-
JNE37_RS09260	1957033..1957428	-	396	WP_203066041.1	PIN domain-containing protein	Toxin
JNE37_RS09265	1957418..1957648	-	231	WP_203066042.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JNE37_RS09270	1957709..1957909	-	201	WP_203066043.1	DUF1674 domain-containing protein	-
JNE37_RS09275	1958021..1959010	+	990	WP_203066360.1	zinc metalloprotease HtpX	-
JNE37_RS09280	1959017..1960324	+	1308	WP_203066044.1	RsmB/NOP family class I SAM-dependent RNA methyltransferase	-
JNE37_RS09285	1960403..1962049	+	1647	WP_203066045.1	heparinase II/III family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-117)

Antitoxin

• SpoVT-AbrB domain (5-43)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14154.33 Da
• Isoelectric Point: 4.6652

>T188816 WP_203066041.1 NZ_CP068983:c1957428-1957033 [Paradevosia shaoguanensis]
MSGSFIDTNILLYALSADRQKADRAEAVLSAGGIVSVQVLNEFAAVARRKLGLPYPEIAYLLATYRGLVEVRPLTVEIHL
AGLALAGEHDLSIYDAMIVASALEAGCDVLYSEDMQAGRVFEGRLRLVNPF

Nucleotide

• Length: 396 bp

>T188816 NZ_CP090570:765921-766217 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8732.00 Da
• Isoelectric Point: 5.8607

>AT188816 WP_203066042.1 NZ_CP068983:c1957648-1957418 [Paradevosia shaoguanensis]
MLVSKWGNSLAVRLPASVVDALDLKEGDEVTLRIEDERDFVVERKPGRAQLLERLRKHRGILPADFVFDRDCANER

Nucleotide

• Length: 231 bp

>AT188816 NZ_CP090570:766219-766614 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA