Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 516617..517253 | Replicon | chromosome |
| Accession | NZ_CP068982 | ||
| Organism | Bacillus subtilis strain pb2441 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | JNE32_RS02645 | Protein ID | WP_003156187.1 |
| Coordinates | 516903..517253 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | G4NU32 |
| Locus tag | JNE32_RS02640 | Protein ID | WP_003225183.1 |
| Coordinates | 516617..516898 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JNE32_RS02620 (512977) | 512977..513576 | - | 600 | WP_003246687.1 | rhomboid family intramembrane serine protease | - |
| JNE32_RS02625 (513671) | 513671..514036 | + | 366 | WP_015252768.1 | holo-ACP synthase | - |
| JNE32_RS02630 (514202) | 514202..515218 | + | 1017 | WP_072557167.1 | outer membrane lipoprotein carrier protein LolA | - |
| JNE32_RS02635 (515332) | 515332..516501 | + | 1170 | WP_029727188.1 | alanine racemase | - |
| JNE32_RS02640 (516617) | 516617..516898 | + | 282 | WP_003225183.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| JNE32_RS02645 (516903) | 516903..517253 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| JNE32_RS02650 (517369) | 517369..518193 | + | 825 | WP_009966610.1 | RsbT co-antagonist protein RsbRA | - |
| JNE32_RS02655 (518198) | 518198..518563 | + | 366 | WP_250354598.1 | RsbT antagonist protein RsbS | - |
| JNE32_RS02660 (518567) | 518567..518968 | + | 402 | WP_003246640.1 | serine/threonine-protein kinase RsbT | - |
| JNE32_RS02665 (518980) | 518980..519987 | + | 1008 | WP_003234295.1 | phosphoserine phosphatase RsbU | - |
| JNE32_RS02670 (520049) | 520049..520378 | + | 330 | WP_014475877.1 | anti-sigma factor antagonist RsbV | - |
| JNE32_RS02675 (520375) | 520375..520857 | + | 483 | WP_038427419.1 | anti-sigma B factor RsbW | - |
| JNE32_RS02680 (520823) | 520823..521611 | + | 789 | WP_003246715.1 | RNA polymerase sigma factor SigB | - |
| JNE32_RS02685 (521611) | 521611..522210 | + | 600 | WP_250354599.1 | phosphoserine phosphatase RsbX | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T188806 WP_003156187.1 NZ_CP068982:516903-517253 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T188806 NZ_CP090568:2266767-2267018 [Xanthomonas citri pv. malvacearum]
ATGCAACGGCTGCGCGAATTCCTGAAGCCGAAGAATCCCGATGCCGCGAGGCGGGCCGGTGAAGCGATTAGGCGCGGCGT
CCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAGAAGATCTGCCTGAACAGTATCGGGAGTGGCTTATCGATT
TTGGGGATAGTGGCTATGTGGCTCGGTACCGCCACGCTGGCGATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAA
GCGGGGTACTGA
ATGCAACGGCTGCGCGAATTCCTGAAGCCGAAGAATCCCGATGCCGCGAGGCGGGCCGGTGAAGCGATTAGGCGCGGCGT
CCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAGAAGATCTGCCTGAACAGTATCGGGAGTGGCTTATCGATT
TTGGGGATAGTGGCTATGTGGCTCGGTACCGCCACGCTGGCGATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAA
GCGGGGTACTGA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10546.92 Da Isoelectric Point: 6.4708
>AT188806 WP_003225183.1 NZ_CP068982:516617-516898 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT188806 NZ_CP090568:2266462-2266740 [Xanthomonas citri pv. malvacearum]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCCGAGGCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGTGAGGCGTTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACGGGCCTGCACGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCGAGCTGGGGA
ACAGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCCGAGGCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGTGAGGCGTTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACGGGCCTGCACGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCGAGCTGGGGA
ACAGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|