Detailed information of TA system TA188805

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 179474..180210
• Replicon: plasmid pfekpn2511-1
• Accession: NZ_CP068973
• Organism: Klebsiella pneumoniae strain fekpn2511

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: JN237_RS26230
• Protein ID: WP_004187044.1
• Coordinates: 179474..179956 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: JN237_RS26235
• Protein ID: WP_003026799.1
• Coordinates: 179944..180210 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN237_RS26205	176005..176941	-	937	Protein_181	ISNCY family transposase	-
JN237_RS26210	177051..177221	+	171	Protein_182	transposase	-
JN237_RS26215	177219..177326	+	108	Protein_183	IS3 family transposase	-
JN237_RS26220	177474..177872	-	399	WP_032422684.1	helix-turn-helix domain-containing protein	-
JN237_RS26225	177921..179267	-	1347	WP_077254728.1	ISNCY family transposase	-
JN237_RS26230	179474..179956	-	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
JN237_RS26235	179944..180210	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
JN237_RS26240	181322..182131	-	810	WP_023329017.1	N-formylglutamate deformylase	-
JN237_RS26245	182124..183332	-	1209	WP_032448288.1	imidazolonepropionase	-
JN237_RS26250	183344..184738	-	1395	WP_032439652.1	cytosine permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaLAP-2 / qnrS1 / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2	iutA / iucD / iucC / iucB / iucA	1..200198	200198

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T188805 WP_004187044.1 NZ_CP068973:c179956-179474 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T188805 NZ_CP090568:487739-487873 [Xanthomonas citri pv. malvacearum]
ATGAAGCGTGCAATTGTGCTGCTGGTGTTATCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT188805 WP_003026799.1 NZ_CP068973:c180210-179944 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT188805 NZ_CP090568:487512-487664 [Xanthomonas citri pv. malvacearum]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGACGGCGTGATGACGGGCTGCAACACCGTGGCCGGCGC
CGGCAAGGACATGCAGGGTGCGGGCAACAAGGTCGAGAAAACGGCTGAAAAATGCAGCGACGGTAAGTGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66