Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4554359..4555169 Replicon chromosome
Accession NZ_CP068972
Organism Klebsiella pneumoniae strain fekpn2511

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag JN237_RS22160 Protein ID WP_004178461.1
Coordinates 4554359..4554892 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JN237_RS22165 Protein ID WP_002887278.1
Coordinates 4554903..4555169 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN237_RS22155 4553190..4554311 + 1122 WP_002887282.1 cupin domain-containing protein -
JN237_RS22160 4554359..4554892 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
JN237_RS22165 4554903..4555169 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
JN237_RS22170 4555272..4556705 - 1434 WP_040216929.1 Cu(+)/Ag(+) sensor histidine kinase -
JN237_RS22175 4556695..4557378 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
JN237_RS22180 4557551..4558936 + 1386 WP_040216925.1 efflux transporter outer membrane subunit -
JN237_RS22185 4558954..4559298 + 345 WP_025862174.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T188802 WP_004178461.1 NZ_CP068972:c4554892-4554359 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T188802 NZ_CP090568:219352-219678 [Xanthomonas citri pv. malvacearum]
ATGATGCGGGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAAAACCACGGCCCGCTCTGGTGATCCAGGCCGA
CCAGTTTGACGCACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTCGTTGCTGCGCCGCTGCTGCGCATTACCA
TTCATCCAAGTACCGACAATGGTTTGCAGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
GTAGGTCGAGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGAAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT188802 WP_002887278.1 NZ_CP068972:c4555169-4554903 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT188802 NZ_CP090568:219185-219355 [Xanthomonas citri pv. malvacearum]
ATGGCAGGGCTGCGTCCGGTGCAAATTTGGGTGCCGGACACACGGCGGCCTGACTTCGCCGAGGAATGCCGCCGTCAGTG
TCGTCTTGCTGCACAAGCGGACATGGCGGACACCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCT
GGACGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References