Detailed information of TA system TA188790

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 1753079..1753722
• Replicon: chromosome
• Accession: NZ_CP068972
• Organism: Klebsiella pneumoniae strain fekpn2511

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A4GZE5
• Locus tag: JN237_RS08440
• Protein ID: WP_015874977.1
• Coordinates: 1753079..1753495 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A4GZE4
• Locus tag: JN237_RS08445
• Protein ID: WP_015874976.1
• Coordinates: 1753492..1753722 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN237_RS08425	1748482..1749597	-	1116	WP_032447702.1	salmochelin biosynthesis C-glycosyltransferase IroB	-
JN237_RS08430	1750469..1752643	+	2175	WP_015874979.1	siderophore salmochelin receptor IroN	-
JN237_RS08435	1752798..1753043	-	246	WP_032447699.1	hypothetical protein	-
JN237_RS08440	1753079..1753495	-	417	WP_015874977.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN237_RS08445	1753492..1753722	-	231	WP_015874976.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JN237_RS08450	1754165..1754545	+	381	WP_015874975.1	YrzE family protein	-
JN237_RS08455	1754682..1755635	+	954	Protein_1653	IS21 family transposase	-
JN237_RS08460	1755730..1756456	+	727	Protein_1654	IS21-like element IS100kyp family helper ATPase IstB	-
JN237_RS08465	1756500..1756613	-	114	WP_015874970.1	hypothetical protein	-
JN237_RS08470	1756856..1757065	-	210	WP_032447696.1	TraR/DksA family transcriptional regulator	-
JN237_RS08475	1757055..1757636	-	582	WP_000937857.1	DUF2857 domain-containing protein	-
JN237_RS08480	1757621..1757803	-	183	WP_072001695.1	hypothetical protein	-
JN237_RS08485	1757825..1758010	-	186	WP_000205185.1	AlpA family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	rmpA / iroD / iroC / iroB / iroN	1714810..1759076	44266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14945.39 Da
• Isoelectric Point: 8.5464

>T188790 WP_015874977.1 NZ_CP068972:c1753495-1753079 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAGLRGDRIVVSAVTYAEMRFGATGPKASPHHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T188790 NZ_CP090562:35905-36237 [Xanthomonas citri pv. malvacearum]
ATGGATCGCGGCGATGTCTATTTGGTTTCGCTCGATCCCACGTCCGGCCATGAACAACAGGGCACGCGCCCGGTGCTGAT
TGTGTCGTCAAGCGCGTTCAACCGCCTGACGAAAACACCGGTCGTGCTGCCGATCACCAGTGGGGGTAACTTCGCCCGCA
CGGCAGGCTTTACCGTGTCGCTCATGGGTGCGGGTACCAACACAACTGGCGTAGTTCGCTGCGATCAGCCCCGCGCTCTC
GATCTAGCCTCGCGGCGCGGGCGCAAGCTGGAAACCGTGCCGTCCCCGATCATGGATGAGGTATTAGCGAAGCTGAGCGC
AATCCTTGAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8749.80 Da
• Isoelectric Point: 4.3533

>AT188790 WP_015874976.1 NZ_CP068972:c1753722-1753492 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMVEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT188790 NZ_CP090562:35648-35905 [Xanthomonas citri pv. malvacearum]
ATGCATACGACCAATCTGCGTAAGGTCGGTGGCTCGATCATGTTGGCTGTCCCGCCCGCCATTCTGGATATGCTGCGTCT
GCGTGCCGGCGCTACCGTTGGCTTGGCCGTCGATCATGGTCGCCTGGTGATCGAACCCACGCTGCGTCCGCACTACAGCC
TGGATGAGCTGCTGGTCCAATGCGACGCATCCGCCGAACTCTCCACCGAAGATCGGGAATGGCTCGACGCCAAGCCTGTT
GGCAGCGAGCTGCTGTGA

Structures

Toxin

• AlphaFold DB: A4GZE5

Antitoxin

• AlphaFold DB: A4GZE4