Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4784256..4784753 Replicon chromosome
Accession NZ_CP068830
Organism Escherichia coli strain RIVM_C018707

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JNN82_RS23165 Protein ID WP_001173343.1
Coordinates 4784256..4784429 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JNN82_RS23170 Protein ID WP_000398619.1
Coordinates 4784457..4784753 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN82_RS23155 4780926..4782383 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
JNN82_RS23160 4782620..4784137 + 1518 WP_001550472.1 lysine--tRNA ligase -
JNN82_RS23165 4784256..4784429 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JNN82_RS23170 4784457..4784753 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JNN82_RS23175 4784982..4785254 - 273 WP_000405647.1 N-acetyltransferase -
JNN82_RS23180 4785266..4785496 - 231 WP_001550470.1 (4Fe-4S)-binding protein -
JNN82_RS23185 4785676..4787307 + 1632 WP_001216477.1 sensor histidine kinase -
JNN82_RS23190 4787304..4788023 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4739379..4784753 45374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T188786 WP_001173343.1 NZ_CP068830:c4784429-4784256 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T188786 NZ_CP090561:483235-483387 [Xanthomonas citri pv. malvacearum]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGTAGGCGTGATGACGGGCTGCAACACCGTGGCCGGCGC
CGGCAAGGACATGCAGGGTGCGGGCAACAAGGTCGAGAAAACGTCCGAAAAATGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT188786 WP_000398619.1 NZ_CP068830:c4784753-4784457 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT188786 NZ_CP090561:483462-483596 [Xanthomonas citri pv. malvacearum]
ATGAAGCGTGCAATTGTGCTGCTGGTGTTATCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References