Detailed information of TA system TA188785

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4772669..4773503
• Replicon: chromosome
• Accession: NZ_CP068830
• Organism: Escherichia coli strain RIVM_C018707

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: JNN82_RS23120
• Protein ID: WP_000854820.1
• Coordinates: 4773126..4773503 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: JNN82_RS23115
• Protein ID: WP_001285610.1
• Coordinates: 4772669..4773037 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN82_RS23075	4768390..4769070	+	681	WP_021515201.1	WYL domain-containing protein	-
JNN82_RS23080	4769221..4769898	+	678	WP_060579144.1	hypothetical protein	-
JNN82_RS23085	4769904..4770137	+	234	WP_000883181.1	DUF905 family protein	-
JNN82_RS23090	4770227..4771045	+	819	WP_001175155.1	DUF945 domain-containing protein	-
JNN82_RS23095	4771071..4771211	-	141	WP_000997937.1	hypothetical protein	-
JNN82_RS23100	4771311..4771790	+	480	WP_000706978.1	antirestriction protein	-
JNN82_RS23105	4771805..4772281	+	477	WP_001186193.1	RadC family protein	-
JNN82_RS23110	4772368..4772589	+	222	WP_000692347.1	DUF987 domain-containing protein	-
JNN82_RS23115	4772669..4773037	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN82_RS23120	4773126..4773503	+	378	WP_000854820.1	type IV toxin-antitoxin system toxin CbtA	Toxin
JNN82_RS23125	4773500..4773697	+	198	Protein_4529	hypothetical protein	-
JNN82_RS23130	4773725..4773922	+	198	WP_085949158.1	DUF957 domain-containing protein	-
JNN82_RS23135	4774007..4774567	+	561	Protein_4531	DUF4942 domain-containing protein	-
JNN82_RS23140	4775402..4776940	+	1539	WP_001187178.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4739379..4784753	45374

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T188785 WP_000854820.1 NZ_CP068830:4773126-4773503 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T188785 NZ_CP090561:c221975-221694 [Xanthomonas citri pv. malvacearum]
ATGCGGACGATTGACCGTTCATCCGCTTTCAAGCGCGACTACAAGCGGGAAGCCAAGGGGCAGCATCGCGCCACGCTTGA
TGACGCGATCAAGCCGGTGTTGGTCGCACTGGCGACCGATCAGCCTCCCGATGCACGCTATCGCGATCACGATCTTTCCG
GCGATTGGGCGGGCTATCGGGAATGTCACATCAAGCCCGACTTGCTGCTGATCTACCGCAAGTCCGACCCCGACACCCTG
CGACTGGCACGGCTTGGCTCGCACAGCGAGCTTTTCGGCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT188785 WP_001285610.1 NZ_CP068830:4772669-4773037 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT188785 NZ_CP090561:c222231-221962 [Xanthomonas citri pv. malvacearum]
ATGAGCACTACCGCCGATACCTACGTCCGCGCCCGCATCGACACCGCCACCAAGGAACGTGCCGCTAGTGCGCTCGAAGC
AATGGGGCTGTCGATCTCTGATGCTATCCGCCTGCTGATGCTACGCATTGCCGACGAACACCGCCTGCCCTTCGATGTGA
AGGTACCAAACACCACCACGAAAAAGGCGATTGCCGAACTGGAATCAGGCAAGGGTAAGAAGTTCGCCAATGTGGACGAC
CTGATGGCGGATTTGCATGCGGACGATTGA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6