Detailed information of TA system TA188776

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3154251..3155035
• Replicon: chromosome
• Accession: NZ_CP068830
• Organism: Escherichia coli strain RIVM_C018707

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: JNN82_RS15305
• Protein ID: WP_000613626.1
• Coordinates: 3154541..3155035 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: JNN82_RS15300
• Protein ID: WP_001110447.1
• Coordinates: 3154251..3154544 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN82_RS15290	3149401..3150360	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
JNN82_RS15295	3150933..3154118	+	3186	WP_001550951.1	ribonuclease E	-
JNN82_RS15300	3154251..3154544	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
JNN82_RS15305	3154541..3155035	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
JNN82_RS15310	3155130..3156083	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
JNN82_RS15315	3156095..3157738	-	1644	WP_000096478.1	flagellar hook-associated protein FlgK	-
JNN82_RS15320	3157804..3158745	-	942	WP_001366226.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
JNN82_RS15325	3158745..3159842	-	1098	WP_001441925.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T188776 WP_000613626.1 NZ_CP068830:3154541-3155035 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T188776 NZ_CP090558:c138382-137744 [Enterococcus faecalis]
ATGATACTAAATAAGAAAAAATTATATTTCGAAACACAGGCATTTACTGACAAACTTATAAATGAAATTTCAATCAATAT
GTCTAAAAGAAAAGAACTCATCAATTGTTATGATATTGAAGCCTACGTAAAAGAAACCGAAAACATTGATTTTTTTGATT
ATCCATTCAAAAAACAATCTAAAAAATTAATCTGGGGATCAGTAACAAAAATTGGTGATGTAACTATGGTAGCGACTGCC
CAACACTTGAAAATAGAAATTAAAAATTTTACGAAAATGCACGAAATAATTCATCATTATTTTGACTATACGACAGATAT
GCCTAACAATGAAAAATCATTTGATACACTACTTATAAAAAAGGGCTATCTTCCTGAAGATTATCCTAAAGAATTCAGAG
CAAATAAGGGTGCCGGAATGTTACTTATTAATAAAAAAGCCTTAAAGTATGCATTTAGTACTTATGATGATCTTGAAAAA
ATGGCTAGTTTTTTCAACGTAAACGATATAGTTCTCAAGATAAGAATTCTTGACGAATTAATCTATTCCCACAACTTATC
TTATAAACAAGCCAATCTTATTTATAATAGTTACTATTTTCAACAACAAACACAACTTAAAGAGATTTTACTCAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT188776 WP_001110447.1 NZ_CP068830:3154251-3154544 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT188776 NZ_CP090558:c138763-138389 [Enterococcus faecalis]
ATGAAAACTTACGAAAAGATAAAACAATTAAGAGAAAATAGAGAAATCACTCAAAAAGAAATTTCAAACGCACTAGACAT
TAATGTTAGTGTATATAATAAAATAGAACTTGGAATACGTCCATTAAGAGAAGAAGAACTTACTGCTATTGCTGATTTTT
TTAATGTTTCAATTGATTATTTAACAGGACGCACTGATAACCCTACAACACCTTCAAATAATCAAAAAGAAGCTGGACAA
AATATTATTTCACATTTCAGATTAAACACTAATGATTTAAGTTCTGATGACATAGAAGAATTAGAAAAAGAATTGATTGA
TTTTCAAGAATTTCTTATAAAACGAGCAAAAGAAAGAAAAAAAAAGAGTGAGTAA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6