Detailed information of TA system TA188775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2723780..2724418
• Replicon: chromosome
• Accession: NZ_CP068830
• Organism: Escherichia coli strain RIVM_C018707

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: JNN82_RS13095
• Protein ID: WP_000813795.1
• Coordinates: 2724242..2724418 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JNN82_RS13090
• Protein ID: WP_076797675.1
• Coordinates: 2723780..2724196 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN82_RS13070	2718932..2719873	-	942	WP_001251315.1	ABC transporter permease	-
JNN82_RS13075	2719874..2720887	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
JNN82_RS13080	2720905..2722050	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
JNN82_RS13085	2722295..2723701	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
JNN82_RS13090	2723780..2724196	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JNN82_RS13095	2724242..2724418	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JNN82_RS13100	2724640..2724870	+	231	WP_023910283.1	YncJ family protein	-
JNN82_RS13105	2724962..2726923	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JNN82_RS13110	2726996..2727532	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
JNN82_RS13115	2727585..2728796	+	1212	WP_071591517.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T188775 WP_000813795.1 NZ_CP068830:c2724418-2724242 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T188775 NZ_CP090556:c2315406-2315062 [Actinobacillus suis]
ATGAATTCTGATGAATATTTAACCTTTGTTGAGGTTGGTACATTCGAGGAAGATCGTAAGCAATTAATGGCAGACGATGA
GTACCAATTATTCCAAGCATATTTATTAGAGCATTACGAATTAGGGGATTTTATTTCCCATACAGGTGGTTGCCAAAAGA
TCAGGTGGCGCTTACCGGAAAATAATAAGGGGAAAAGTGCTGGTGTTCGAATTATCTATTATGTTAGAAGTTTAACAGGG
CGAATTTACCTAATTACTATGTATGGAAAAAGCGAAAAAGTTAATCTAAATGCTCGAGAAAAAGCGATTATGAAGAAAAT
TATTTCGAGATTAATAGGAGAGTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT188775 WP_076797675.1 NZ_CP068830:c2724196-2723780 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT188775 NZ_CP090556:c2315057-2314761 [Actinobacillus suis]
ATGGAAAAACGTTTATTTGAGCGCCTTGTACAAGGATTAGAAGAAATGGTTGCTATTGAAAAAGGTGAGGTGGAAGTACC
ATCGCATCGCATCCATGCACATAAAATTCCAAATACTAAGGCGATTCGTACACAACACCATTTGAAACAGGCAGAATTTG
CACAGCTGTTAGGTGTCAGTACCAATTTGGTGCAATCTTGGGAAATCGGCAGACGAGTCCCTAATGGAGCGGCAAGAAAA
TTATTGGCGATTATCGAACAACAACCGAATTTAGTCCCTTTGCTACAATCTCTCTAA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1