Detailed information of TA system TA188774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2619067..2620655
• Replicon: chromosome
• Accession: NZ_CP068830
• Organism: Escherichia coli strain RIVM_C018707

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A829LC11
• Locus tag: JNN82_RS12655
• Protein ID: WP_001551128.1
• Coordinates: 2619333..2620655 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JNN82_RS12650
• Protein ID: WP_001296726.1
• Coordinates: 2619067..2619333 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN82_RS12650	2619067..2619333	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JNN82_RS12655	2619333..2620655	+	1323	WP_001551128.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
JNN82_RS12660	2620855..2622207	-	1353	WP_001551127.1	SIR2 family protein	-
JNN82_RS12665	2622422..2623454	-	1033	Protein_2479	ISNCY family transposase	-
JNN82_RS12670	2623821..2624720	-	900	WP_001551125.1	LysR family transcriptional regulator	-
JNN82_RS12675	2624859..2625167	+	309	WP_001221046.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2622422..2623240	818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49270.88 Da
• Isoelectric Point: 8.2472

>T188774 WP_001551128.1 NZ_CP068830:2619333-2620655 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGVNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T188774 NZ_CP090556:1578899-1580221 [Actinobacillus suis]
ATGGTGAAGTGTTTATGTGTGTTAATGAATGGGGAATTAGTTGGTTTTTTTGAGCGTACTATGTCCGGTTCGCATTTATT
CCGTTATGCAGATGAGTGGTTTTTATCTCCAAATGTTAGACCTATTTCTCTTTCTATGCCATTAAGAAGGGAGGTTTATC
AAGGTAAAGAAGTAATTAATTTTTTTGATAACTTATTACCTGATCACCCTCAAGTTAGAGCTAGAATTGCTGCGCGATTT
CAAGCTGAAACACAACAACCTTTTGAATTGTTAGCTAAGATTGGACGAGATGCAACCGGAGCAATAACCCTATTAGAAGA
AAACACAGAAATTCCCAATCATAAAGTTATTTATGCAACGGAATTAAACGAAAATGAACTTCATTCGTTATTATTAGGCT
ATCAGCAAAATGCACCACTTGGCATGTTGGATGAAGCACAAGATTTTCGAATTTCTATCGCAGGGGCTCAAGAAAAGACA
GCATTATTATGGTATAACGAACGTTGGCAATTACCCCATGGATTAACCCCAACGACACATATTATTAAATTACCGATAGG
TAAAATACAAACACCTAAATATCAATTAGATCTGTCTCAAAGTGTAGACAACGAATTAATTTGTTTACGCTTATGTGCTG
CCTTCGGTTTACCGACTGCAAATGTAAATTTATTAGAGGTCGGGGATGTGCGAGCATTGGTTGTAGAACGGTTTGATCGT
AAATTACATTCTTCAAATAAATATTTTTTACGCTTACCTTATGAAGATTTTTGCCAAGTATTCGGCTTAGCGCCAACCCA
AAAATATGAGAGTGATGGTGGTATCGGTATTCGACACATTATGGATAAACTTTTGTATTCCAGTCATACTTCGGATCGTG
CACAATTCATGAAAACCCAATTTTTATTTTGGGTGCTTGGAGCAATAGATGGGCATGCCAAAAACTTTTCTATTGCATTG
AAGTCGCAGAGTCGTTATCACTTATCGCCAATTTATGATGTTATATCGGCTTATCCAATGCTGGGAGGTAAAGGATGGCA
TCAGAGTGATTTGCAACTTGCGATGAGTCTGTATAGTACCCAAAAAGGTAAGAAATGGAGTTGGCAAAAAATACGACCGG
TACATTATCTGTCTACCGCTAAATCTGTCGGATTTAATCAGTTAGAAATGAAACATATTATTGAAGAGATTTCAGATACG
GCATTAAAAAAGTTAGATTTTGTAGCTGATAGGCTGCCAATAAATATTAATGAACAAGTAAGAGATCAAATTTTTAATGG
AGTCAAAAAACAGATTGATAAAATCAAATTAGATAGGATTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT188774 WP_001296726.1 NZ_CP068830:2619067-2619333 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT188774 NZ_CP090556:1578663-1578905 [Actinobacillus suis]
ATGCTAATCAATGATCCTAATAGTTTAGCCGTTTACCTACGTGATATTCGTAAGCAGCAGAAACAATCACAAAAAATGAT
TGCTCAACGAGTCGGTTTGCGACAAGCAACGATTTCTACATTCGAGGCCCAGCCTGATAAAGTCACAATAGAAACGCTAT
TTAAAATAGTTCATTCTCTTGGTTTAGAAATTCATTTAGAAGAAAAGCATACGAATATGTTTAATAGGAGCGATGAATGG
TGA

Structures

Toxin

• AlphaFold DB: A0A829LC11