Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4708880..4709377 Replicon chromosome
Accession NZ_CP068829
Organism Escherichia coli strain RIVM_C030453

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JNN50_RS22715 Protein ID WP_001173343.1
Coordinates 4708880..4709053 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JNN50_RS22720 Protein ID WP_000398619.1
Coordinates 4709081..4709377 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN50_RS22705 4705550..4707007 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
JNN50_RS22710 4707244..4708761 + 1518 WP_001550472.1 lysine--tRNA ligase -
JNN50_RS22715 4708880..4709053 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JNN50_RS22720 4709081..4709377 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JNN50_RS22725 4709606..4709878 - 273 WP_000405647.1 N-acetyltransferase -
JNN50_RS22730 4709890..4710120 - 231 WP_001550470.1 (4Fe-4S)-binding protein -
JNN50_RS22735 4710300..4711931 + 1632 WP_001216477.1 sensor histidine kinase -
JNN50_RS22740 4711928..4712647 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T188756 WP_001173343.1 NZ_CP068829:c4709053-4708880 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T188756 NZ_CP090547:c1519932-1519540 [Xylella fastidiosa subsp. sandyi]
ATGCTCGACACAAATATCATCAGCGATATCATTCGTAACCCATTCGGGGCGGTGGCTTGCCGTATTGAACACGTTGGGTA
TCCGAACATTTGCACGAGCGTTATCGTGGCGGCCGAACTTCGCTATGGATGCACGAAAAATGGCTCGGTAAAGCTGCTGA
GCAGGGTACAGGACATTCTTAAAACGCTGCCGATCCTGCCTTTGGACATACCTGTTGACACAACATATGGCAGTATCCGT
GCTGAGCTGGAGGCTGCGGGTCAGCTGATCGGCGCTAATGATCTATTGATTGCTGCGCATGCTTACGTACTCGGGCTGAC
TTTGGTCACGGACAACACCCGCGAGTTCAGCCGAATTCGCGGCTTGGATGTGCAAAACTGGCTAGAGAGGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT188756 WP_000398619.1 NZ_CP068829:c4709377-4709081 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT188756 NZ_CP090547:c1520213-1519941 [Xylella fastidiosa subsp. sandyi]
ATGCCAATTTCATCTCCGTTATCACGTACTAAGGAAGCGAAGCTATTCCGAAATAATCGTAGCCAGGCGGTACGAATTCC
CGTTGAATTCGAGCTGTCGGGCGATCGTGTTCTGATCCACCGTGAGGGGAGCAAGCTCATTATTGAGCCGATCACCAGGC
CAACCAATATTGTTGAATTACTGCACGAGTGGCGAAGAGAAACACCACTCGGCCCAGAGGATCAATTCCCTGATATTGAT
GACATGCCCGCTCAGCCAGAGGATATTCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References