Detailed information of TA system TA188752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4349442..4349950
• Replicon: chromosome
• Accession: NZ_CP068829
• Organism: Escherichia coli strain RIVM_C030453

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: JNN50_RS20835
• Protein ID: WP_001534843.1
• Coordinates: 4349741..4349950 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0V9L4L3
• Locus tag: JNN50_RS20830
• Protein ID: WP_000063161.1
• Coordinates: 4349442..4349744 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN50_RS20815	4345970..4348120	+	2151	WP_001366791.1	pyruvate/proton symporter BtsT	-
JNN50_RS20820	4348238..4348441	+	204	WP_001534846.1	YbdD/YjiX family protein	-
JNN50_RS20825	4348452..4349408	+	957	WP_001534845.1	GTPase	-
JNN50_RS20830	4349442..4349744	-	303	WP_000063161.1	BrnA antitoxin family protein	Antitoxin
JNN50_RS20835	4349741..4349950	-	210	WP_001534843.1	BrnT family toxin	Toxin
JNN50_RS20840	4350046..4351212	+	1167	WP_001534841.1	restriction endonuclease	-
JNN50_RS20845	4351276..4352895	+	1620	WP_001550561.1	type I restriction-modification system subunit M	-
JNN50_RS20850	4352885..4354195	+	1311	WP_001534837.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7952.17 Da
• Isoelectric Point: 4.9493

>T188752 WP_001534843.1 NZ_CP068829:c4349950-4349741 [Escherichia coli]
MVDYPKRDAISNINAIIVDTFLYIQLGMATMPMEFEWNANKAKSNRVKHGIRFEDAVLVLDDPQRTEPL

Nucleotide

• Length: 210 bp

>T188752 NZ_CP090547:c1056801-1056409 [Xylella fastidiosa subsp. sandyi]
ATGCTCGACACAAATATCATCAGCGATATCATTCGTAACCCATTCGGGGCGGTGGCTTGCCGTATTGAACACGTTGGGTA
TCCGAACATTTGCACGAGCGTTATCGTGGCGGCCGAACTTCGCTATGGATGCACGAAAAATGGCTCGGTAAAGCTGCTGA
GCAGGGTACAGGACATTCTTAAAACGCTGCCGATCCTGCCTTTGGACATACCTGTTGACACAACATATGGCAGTATCCGT
GCTGAGCTGGAGGCTGCGGGTCAGCTGATCGGCGCTAATGATCTATTGATTGCTGCGCATGCTTACGTACTCGGGCTGAC
TTTGGTCACGGACAACACCCGCGAGTTCAGCCGAATTCGCGGCTTGGATGTGCAAAACTGGCTAGAGAGGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11600.10 Da
• Isoelectric Point: 9.4646

>AT188752 WP_000063161.1 NZ_CP068829:c4349744-4349442 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKVLANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT188752 NZ_CP090547:c1057082-1056810 [Xylella fastidiosa subsp. sandyi]
ATGCCAATTTCATCTCCGTTATCACGTACTAAGGAAGCGAAGCTATTCCGAAATAATCGTAGCCAGGCGGTACGAATTCC
CGTTGAATTCGAGCTGTCGGGCGATCGTGTTCTGATCCACCGTGAGGGGAGCAAGCTCATTATTGAGCCGATCACCAGGC
CAACCAATATTGTTGAATTACTGCACGAGTGGCGAAGAGAAACACCACTCGGCCCAGAGGATCAATTCCCTGATATTGAT
GACATGCCCGCTCAGCCAGAGGATATTCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0V9L4L3