Detailed information of TA system TA188748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3385933..3386638
• Replicon: chromosome
• Accession: NZ_CP068829
• Organism: Escherichia coli strain RIVM_C030453

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: JNN50_RS16440
• Protein ID: WP_000539521.1
• Coordinates: 3385933..3386319 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JNN50_RS16445
• Protein ID: WP_001280945.1
• Coordinates: 3386309..3386638 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN50_RS16420	3381937..3382563	+	627	WP_001295292.1	glutathione S-transferase GstB	-
JNN50_RS16425	3382560..3383675	-	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
JNN50_RS16430	3383786..3384169	-	384	WP_000497137.1	biofilm formation regulator BssR	-
JNN50_RS16435	3384382..3385707	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
JNN50_RS16440	3385933..3386319	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JNN50_RS16445	3386309..3386638	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
JNN50_RS16450	3386708..3388036	-	1329	WP_001550887.1	GGDEF domain-containing protein	-
JNN50_RS16455	3388044..3390392	-	2349	WP_001550886.1	EAL domain-containing protein	-
JNN50_RS16460	3390570..3391481	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T188748 WP_000539521.1 NZ_CP068829:3385933-3386319 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T188748 NZ_CP090545:c4823509-4823198 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT188748 WP_001280945.1 NZ_CP068829:3386309-3386638 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT188748 NZ_CP090545:c4823197-4822907 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6