Detailed information of TA system TA188727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4366126..4366940
• Replicon: chromosome
• Accession: NZ_CP068828
• Organism: Escherichia coli strain RIVM_C030443

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: JNN35_RS20895
• Protein ID: WP_001054376.1
• Coordinates: 4366126..4366383 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: JNN35_RS20900
• Protein ID: WP_001309181.1
• Coordinates: 4366395..4366940 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN35_RS20875	4361414..4362520	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
JNN35_RS20880	4362585..4363565	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
JNN35_RS20885	4364148..4365388	-	1241	Protein_4089	helicase YjhR	-
JNN35_RS20890	4365504..4365749	+	246	Protein_4090	GNAT family N-acetyltransferase	-
JNN35_RS20895	4366126..4366383	+	258	WP_001054376.1	hypothetical protein	Toxin
JNN35_RS20900	4366395..4366940	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
JNN35_RS20905	4366996..4367742	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
JNN35_RS20910	4367911..4368129	+	219	Protein_4094	hypothetical protein	-
JNN35_RS20915	4368167..4368283	+	117	Protein_4095	VOC family protein	-
JNN35_RS20920	4368528..4369649	+	1122	WP_000010829.1	M42 family metallopeptidase	-
JNN35_RS20925	4369646..4369924	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
JNN35_RS20930	4369936..4371249	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	4355912..4375164	19252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T188727 WP_001054376.1 NZ_CP068828:4366126-4366383 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T188727 NZ_CP090541:3499510-3499728 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT188727 WP_001309181.1 NZ_CP068828:4366395-4366940 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT188727 NZ_CP090541:3499108-3499482 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8