Detailed information of TA system TA188723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3299390..3300095
• Replicon: chromosome
• Accession: NZ_CP068828
• Organism: Escherichia coli strain RIVM_C030443

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: JNN35_RS15930
• Protein ID: WP_000539521.1
• Coordinates: 3299390..3299776 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JNN35_RS15935
• Protein ID: WP_001280945.1
• Coordinates: 3299766..3300095 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN35_RS15910	3295394..3296020	+	627	WP_001295292.1	glutathione S-transferase GstB	-
JNN35_RS15915	3296017..3297132	-	1116	WP_000555038.1	aldose sugar dehydrogenase YliI	-
JNN35_RS15920	3297243..3297626	-	384	WP_000497137.1	biofilm formation regulator BssR	-
JNN35_RS15925	3297839..3299164	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
JNN35_RS15930	3299390..3299776	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JNN35_RS15935	3299766..3300095	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
JNN35_RS15940	3300165..3301493	-	1329	WP_000086879.1	GGDEF domain-containing protein	-
JNN35_RS15945	3301501..3303849	-	2349	WP_001322378.1	EAL domain-containing protein	-
JNN35_RS15950	3304027..3304938	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T188723 WP_000539521.1 NZ_CP068828:3299390-3299776 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T188723 NZ_CP090541:849444-849842 [Salmonella enterica]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT188723 WP_001280945.1 NZ_CP068828:3299766-3300095 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT188723 NZ_CP090541:849217-849444 [Salmonella enterica]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6