Detailed information of TA system TA188721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2695271..2695909
• Replicon: chromosome
• Accession: NZ_CP068828
• Organism: Escherichia coli strain RIVM_C030443

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: JNN35_RS12915
• Protein ID: WP_000813797.1
• Coordinates: 2695733..2695909 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JNN35_RS12910
• Protein ID: WP_076611057.1
• Coordinates: 2695271..2695687 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN35_RS12890	2690423..2691364	-	942	WP_001251335.1	ABC transporter permease	-
JNN35_RS12895	2691365..2692378	-	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
JNN35_RS12900	2692396..2693541	-	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
JNN35_RS12905	2693786..2695192	-	1407	WP_000760613.1	PLP-dependent aminotransferase family protein	-
JNN35_RS12910	2695271..2695687	-	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JNN35_RS12915	2695733..2695909	-	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JNN35_RS12920	2696131..2696361	+	231	WP_000494244.1	YncJ family protein	-
JNN35_RS12925	2696453..2698414	-	1962	WP_001309488.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JNN35_RS12930	2698487..2699023	-	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
JNN35_RS12935	2699076..2700287	+	1212	WP_001323162.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T188721 WP_000813797.1 NZ_CP068828:c2695909-2695733 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T188721 NZ_CP090541:365061-365336 [Salmonella enterica]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT188721 WP_076611057.1 NZ_CP068828:c2695687-2695271 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT188721 NZ_CP090541:364798-365058 [Salmonella enterica]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCCCGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Structures

Toxin

• AlphaFold DB: A0A829J715