Detailed information of TA system TA188719

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2595537..2597125
• Replicon: chromosome
• Accession: NZ_CP068828
• Organism: Escherichia coli strain RIVM_C030443

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: JNN35_RS12470
• Protein ID: WP_001546825.1
• Coordinates: 2595803..2597125 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JNN35_RS12465
• Protein ID: WP_001296726.1
• Coordinates: 2595537..2595803 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN35_RS12465	2595537..2595803	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JNN35_RS12470	2595803..2597125	+	1323	WP_001546825.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
JNN35_RS12475	2597523..2598422	-	900	WP_001057776.1	LysR family transcriptional regulator	-
JNN35_RS12480	2598562..2598870	+	309	WP_001221046.1	hypothetical protein	-
JNN35_RS12485	2598879..2599568	+	690	WP_000801313.1	type 1 glutamine amidotransferase domain-containing protein	-
JNN35_RS12490	2599642..2600589	+	948	WP_000854796.1	aldo/keto reductase	-
JNN35_RS12495	2600692..2600847	+	156	WP_001322415.1	hypothetical protein	-
JNN35_RS12500	2600807..2601226	+	420	WP_001322416.1	amidohydrolase family protein	-
JNN35_RS12505	2601177..2601368	+	192	WP_001469139.1	amidohydrolase family protein	-
JNN35_RS12510	2601519..2602091	+	573	WP_000789913.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49331.00 Da
• Isoelectric Point: 8.1369

>T188719 WP_001546825.1 NZ_CP068828:2595803-2597125 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHCRLSREYGIK

Nucleotide

• Length: 1323 bp

>T188719 NZ_CP090541:206805-207290 [Salmonella enterica]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT188719 WP_001296726.1 NZ_CP068828:2595537-2595803 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT188719 NZ_CP090541:206530-206817 [Salmonella enterica]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA