Detailed information of TA system TA188710

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2045591..2046422
• Replicon: chromosome
• Accession: NZ_CP068828
• Organism: Escherichia coli strain RIVM_C030443

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: JNN35_RS09730
• Protein ID: WP_000854814.1
• Coordinates: 2045591..2045965 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B3Y195
• Locus tag: JNN35_RS09735
• Protein ID: WP_001285585.1
• Coordinates: 2046054..2046422 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN35_RS09690	2040987..2042153	+	1167	WP_000830150.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
JNN35_RS09695	2042272..2042745	+	474	WP_001105389.1	DNA gyrase inhibitor SbmC	-
JNN35_RS09700	2042943..2044001	+	1059	WP_001200891.1	FUSC family protein	-
JNN35_RS09705	2044173..2044502	+	330	WP_000450409.1	DUF496 family protein	-
JNN35_RS09710	2044603..2044785	-	183	WP_001016348.1	hypothetical protein	-
JNN35_RS09715	2044857..2044985	+	129	Protein_1902	transposase domain-containing protein	-
JNN35_RS09720	2045274..2045387	-	114	WP_001161660.1	DUF957 domain-containing protein	-
JNN35_RS09725	2045400..2045594	-	195	WP_000988600.1	hypothetical protein	-
JNN35_RS09730	2045591..2045965	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
JNN35_RS09735	2046054..2046422	-	369	WP_001285585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
JNN35_RS09740	2046496..2046717	-	222	WP_000692323.1	DUF987 domain-containing protein	-
JNN35_RS09745	2046780..2047256	-	477	WP_001186726.1	RadC family protein	-
JNN35_RS09750	2047272..2047751	-	480	WP_000860087.1	antirestriction protein	-
JNN35_RS09755	2047833..2048651	-	819	WP_001234629.1	DUF945 domain-containing protein	-
JNN35_RS09760	2048751..2048984	-	234	WP_001119729.1	DUF905 family protein	-
JNN35_RS09765	2049063..2049518	-	456	WP_000581493.1	hypothetical protein	-
JNN35_RS09770	2049595..2051319	-	1725	Protein_1913	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T188710 WP_000854814.1 NZ_CP068828:c2045965-2045591 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T188710 NZ_CP090539:2534123-2534407 [Salmonella enterica]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.48 Da
• Isoelectric Point: 6.3139

>AT188710 WP_001285585.1 NZ_CP068828:c2046422-2046054 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT188710 NZ_CP090539:2533885-2534133 [Salmonella enterica]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A7U9LW60