Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1019645..1020299 Replicon chromosome
Accession NZ_CP068828
Organism Escherichia coli strain RIVM_C030443

Toxin (Protein)


Gene name cptA Uniprot ID B7N7E0
Locus tag JNN35_RS05000 Protein ID WP_000244769.1
Coordinates 1019892..1020299 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JNN35_RS04995 Protein ID WP_000354046.1
Coordinates 1019645..1019911 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN35_RS04975 1015733..1017166 - 1434 WP_022646090.1 6-phospho-beta-glucosidase BglA -
JNN35_RS04980 1017211..1017522 + 312 WP_001182953.1 N(4)-acetylcytidine aminohydrolase -
JNN35_RS04985 1017686..1018345 + 660 WP_000250269.1 hemolysin III family protein -
JNN35_RS04990 1018422..1019402 - 981 WP_000886049.1 tRNA-modifying protein YgfZ -
JNN35_RS04995 1019645..1019911 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JNN35_RS05000 1019892..1020299 + 408 WP_000244769.1 protein YgfX Toxin
JNN35_RS05005 1020339..1020860 - 522 WP_001055874.1 flavodoxin FldB -
JNN35_RS05010 1020972..1021868 + 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
JNN35_RS05015 1021893..1022603 + 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JNN35_RS05020 1022609..1024342 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16062.03 Da        Isoelectric Point: 11.5554

>T188709 WP_000244769.1 NZ_CP068828:1019892-1020299 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGK
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T188709 NZ_CP090539:2193148-2193251 [Salmonella enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT188709 WP_000354046.1 NZ_CP068828:1019645-1019911 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT188709 NZ_CP090539:c2193253-2193108 [Salmonella enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J3T1

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References