Detailed information of TA system TA188696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3774354..3774862
• Replicon: chromosome
• Accession: NZ_CP068827
• Organism: Escherichia coli strain RIVM_C030371

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: JNN58_RS18060
• Protein ID: WP_001534843.1
• Coordinates: 3774354..3774563 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0V9L4L3
• Locus tag: JNN58_RS18065
• Protein ID: WP_000063161.1
• Coordinates: 3774560..3774862 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN58_RS18045	3770109..3771419	-	1311	WP_001534837.1	restriction endonuclease subunit S	-
JNN58_RS18050	3771409..3773028	-	1620	WP_001550561.1	type I restriction-modification system subunit M	-
JNN58_RS18055	3773092..3774258	-	1167	WP_001534841.1	restriction endonuclease	-
JNN58_RS18060	3774354..3774563	+	210	WP_001534843.1	BrnT family toxin	Toxin
JNN58_RS18065	3774560..3774862	+	303	WP_000063161.1	BrnA antitoxin family protein	Antitoxin
JNN58_RS18070	3774896..3775852	-	957	WP_001534845.1	GTPase	-
JNN58_RS18075	3775863..3776066	-	204	WP_001534846.1	YbdD/YjiX family protein	-
JNN58_RS18080	3776184..3778334	-	2151	WP_001366791.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7952.17 Da
• Isoelectric Point: 4.9493

>T188696 WP_001534843.1 NZ_CP068827:3774354-3774563 [Escherichia coli]
MVDYPKRDAISNINAIIVDTFLYIQLGMATMPMEFEWNANKAKSNRVKHGIRFEDAVLVLDDPQRTEPL

Nucleotide

• Length: 210 bp

>T188696 NZ_CP090535:c4134560-4134273 [Salmonella enterica]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11600.10 Da
• Isoelectric Point: 9.4646

>AT188696 WP_000063161.1 NZ_CP068827:3774560-3774862 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKVLANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT188696 NZ_CP090535:c4134286-4133984 [Salmonella enterica]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A0V9L4L3