Detailed information of TA system TA188681

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 895609..896440
• Replicon: chromosome
• Accession: NZ_CP068827
• Organism: Escherichia coli strain RIVM_C030371

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A6C9I6Z5
• Locus tag: JNN58_RS04375
• Protein ID: WP_001563930.1
• Coordinates: 896066..896440 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B3Y195
• Locus tag: JNN58_RS04370
• Protein ID: WP_001285585.1
• Coordinates: 895609..895977 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN58_RS04340	892498..892953	+	456	WP_000581502.1	hypothetical protein	-
JNN58_RS04345	893032..893265	+	234	WP_001119729.1	DUF905 family protein	-
JNN58_RS04350	893365..894183	+	819	WP_023910202.1	DUF945 domain-containing protein	-
JNN58_RS04355	894274..894759	+	486	WP_085449263.1	antirestriction protein	-
JNN58_RS04360	894775..895251	+	477	WP_202845741.1	RadC family protein	-
JNN58_RS04365	895314..895535	+	222	WP_000692323.1	DUF987 domain-containing protein	-
JNN58_RS04370	895609..895977	+	369	WP_001285585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
JNN58_RS04375	896066..896440	+	375	WP_001563930.1	type IV toxin-antitoxin system toxin CbtA	Toxin
JNN58_RS04380	896437..896631	+	195	WP_000988600.1	hypothetical protein	-
JNN58_RS04385	896644..896757	+	114	WP_001161660.1	DUF957 domain-containing protein	-
JNN58_RS04390	897046..897174	-	129	Protein_864	transposase domain-containing protein	-
JNN58_RS04395	897246..897428	+	183	WP_001016348.1	hypothetical protein	-
JNN58_RS04400	897529..897858	-	330	WP_000450409.1	DUF496 family protein	-
JNN58_RS04405	898030..899088	-	1059	WP_001200891.1	FUSC family protein	-
JNN58_RS04410	899286..899759	-	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
JNN58_RS04415	899878..901044	-	1167	WP_202845742.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13883.97 Da
• Isoelectric Point: 7.8522

>T188681 WP_001563930.1 NZ_CP068827:896066-896440 [Escherichia coli]
MKTLPVLPGLAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T188681 NZ_CP090533:c4473322-4472954 [Salmonella enterica]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.48 Da
• Isoelectric Point: 6.3139

>AT188681 WP_001285585.1 NZ_CP068827:895609-895977 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT188681 NZ_CP090533:c4473540-4473319 [Salmonella enterica]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGGCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A6C9I6Z5

Antitoxin

• AlphaFold DB: A0A7U9LW60