Detailed information of TA system TA188664

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4744489..4745287
• Replicon: chromosome
• Accession: NZ_CP068826
• Organism: Escherichia coli strain RIVM_C030300

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: U9XMP3
• Locus tag: JNN53_RS22900
• Protein ID: WP_000854735.1
• Coordinates: 4744910..4745287 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A067H947
• Locus tag: JNN53_RS22895
• Protein ID: WP_001285415.1
• Coordinates: 4744489..4744863 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN53_RS22860	4740245..4741129	+	885	WP_024166630.1	50S ribosome-binding GTPase	-
JNN53_RS22865	4741248..4741925	+	678	WP_202859610.1	hypothetical protein	-
JNN53_RS22870	4741931..4742164	+	234	WP_001278287.1	DUF905 family protein	-
JNN53_RS22875	4742254..4743072	+	819	WP_001175148.1	DUF945 domain-containing protein	-
JNN53_RS22880	4743154..4743633	+	480	WP_120136233.1	antirestriction protein	-
JNN53_RS22885	4743649..4744125	+	477	WP_001366855.1	RadC family protein	-
JNN53_RS22890	4744188..4744409	+	222	WP_000692309.1	DUF987 domain-containing protein	-
JNN53_RS22895	4744489..4744863	+	375	WP_001285415.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN53_RS22900	4744910..4745287	+	378	WP_000854735.1	TA system toxin CbtA family protein	Toxin
JNN53_RS22905	4745284..4745772	+	489	WP_000761677.1	hypothetical protein	-
JNN53_RS22910	4745784..4745981	+	198	WP_000839291.1	DUF957 domain-containing protein	-
JNN53_RS22915	4746066..4746932	+	867	WP_001280433.1	DUF4942 domain-containing protein	-
JNN53_RS22920	4747004..4747267	+	264	WP_025492088.1	type II toxin-antitoxin system ParD family antitoxin	-
JNN53_RS22925	4747264..4747590	+	327	WP_000779483.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JNN53_RS22930	4748473..4750011	+	1539	WP_001187178.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4727894..4757824	29930

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14061.07 Da
• Isoelectric Point: 8.2904

>T188664 WP_000854735.1 NZ_CP068826:4744910-4745287 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T188664 NZ_CP090529:c4249776-4249492 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13663.32 Da
• Isoelectric Point: 5.8640

>AT188664 WP_001285415.1 NZ_CP068826:4744489-4744863 [Escherichia coli]
VSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT188664 NZ_CP090529:c4250008-4249766 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: U9XMP3

Antitoxin

• AlphaFold DB: A0A067H947