Detailed information of TA system TA188656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3426031..3426736
• Replicon: chromosome
• Accession: NZ_CP068826
• Organism: Escherichia coli strain RIVM_C030300

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: JNN53_RS16660
• Protein ID: WP_000539521.1
• Coordinates: 3426031..3426417 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JNN53_RS16665
• Protein ID: WP_001280945.1
• Coordinates: 3426407..3426736 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN53_RS16640	3422035..3422661	+	627	WP_001295292.1	glutathione S-transferase GstB	-
JNN53_RS16645	3422658..3423773	-	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
JNN53_RS16650	3423884..3424267	-	384	WP_000497137.1	biofilm formation regulator BssR	-
JNN53_RS16655	3424480..3425805	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
JNN53_RS16660	3426031..3426417	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JNN53_RS16665	3426407..3426736	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
JNN53_RS16670	3426806..3428134	-	1329	WP_001550887.1	GGDEF domain-containing protein	-
JNN53_RS16675	3428142..3430490	-	2349	WP_001550886.1	EAL domain-containing protein	-
JNN53_RS16680	3430668..3431579	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T188656 WP_000539521.1 NZ_CP068826:3426031-3426417 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T188656 NZ_CP090529:870362-870775 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT188656 WP_001280945.1 NZ_CP068826:3426407-3426736 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT188656 NZ_CP090529:870115-870381 [Salmonella enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6