Detailed information of TA system TA188624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2360601..2362189
• Replicon: chromosome
• Accession: NZ_CP068825
• Organism: Escherichia coli strain RIVM_C030256

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U9YVT8
• Locus tag: JNN79_RS11355
• Protein ID: WP_001125415.1
• Coordinates: 2360601..2361923 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JNN79_RS11360
• Protein ID: WP_001296726.1
• Coordinates: 2361923..2362189 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN79_RS11315	2355635..2356207	-	573	WP_000789913.1	NAD(P)H-dependent oxidoreductase	-
JNN79_RS11320	2356358..2356549	-	192	WP_001469139.1	amidohydrolase family protein	-
JNN79_RS11325	2356500..2356919	-	420	WP_001322416.1	amidohydrolase family protein	-
JNN79_RS11330	2356879..2357034	-	156	WP_001322415.1	hypothetical protein	-
JNN79_RS11335	2357137..2358084	-	948	WP_000854796.1	aldo/keto reductase	-
JNN79_RS11340	2358158..2358847	-	690	WP_000801313.1	type 1 glutamine amidotransferase domain-containing protein	-
JNN79_RS11345	2358856..2359164	-	309	WP_001221046.1	hypothetical protein	-
JNN79_RS11350	2359304..2360203	+	900	WP_001057776.1	LysR family transcriptional regulator	-
JNN79_RS11355	2360601..2361923	-	1323	WP_001125415.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
JNN79_RS11360	2361923..2362189	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JNN79_RS11365	2362400..2363800	-	1401	WP_001376867.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49384.05 Da
• Isoelectric Point: 8.5830

>T188624 WP_001125415.1 NZ_CP068825:c2361923-2360601 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREYGIK

Nucleotide

• Length: 1323 bp

>T188624 NZ_CP090514:1385210-1385506 [Xylella fastidiosa subsp. fastidiosa]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCGCGTCTTGATCGGCTCGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGTGCTGGCATCTCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATTCTCTTACTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTGTGGAAGGATTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT188624 WP_001296726.1 NZ_CP068825:c2362189-2361923 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT188624 NZ_CP090514:1385510-1385815 [Xylella fastidiosa subsp. fastidiosa]
ATGAATAATGAGACTTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCTGTCGCTCGTGCTCGTAATTTAA
GTCAGCTTGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTAACGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAAGCGCTTGGTTTACGGATGTCATTCACACCTTCCTCTATGAGTTAA

Structures

Toxin

• AlphaFold DB: U9YVT8