Detailed information of TA system TA188617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1867087..1867871
• Replicon: chromosome
• Accession: NZ_CP068825
• Organism: Escherichia coli strain RIVM_C030256

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: JNN79_RS08800
• Protein ID: WP_000613626.1
• Coordinates: 1867087..1867581 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: JNN79_RS08805
• Protein ID: WP_001110447.1
• Coordinates: 1867578..1867871 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN79_RS08780	1862280..1863377	+	1098	WP_000589320.1	flagellar basal body P-ring protein FlgI	-
JNN79_RS08785	1863377..1864318	+	942	WP_001309406.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
JNN79_RS08790	1864384..1866027	+	1644	WP_000096524.1	flagellar hook-associated protein FlgK	-
JNN79_RS08795	1866039..1866992	+	954	WP_202851812.1	flagellar hook-associated protein FlgL	-
JNN79_RS08800	1867087..1867581	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
JNN79_RS08805	1867578..1867871	-	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
JNN79_RS08810	1868004..1871189	-	3186	WP_000827427.1	ribonuclease E	-
JNN79_RS08815	1871762..1872721	+	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T188617 WP_000613626.1 NZ_CP068825:c1867581-1867087 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T188617 NZ_CP090513:c2454516-2454088 [Xylella fastidiosa subsp. multiplex]
ATGATTGTCTTGGATACCAATGTTGTTTCCGAAGCAATGAAACCCGAGCCGGATGCAGCAGTCCGAACCTGGCTCAACGA
GCAGATGAGCGTCACGTTGTACCTATCCAGTGTGACGTTATCTGAACTGTTGTTTGGAATCGCAGTGTTGCCCACAAGTA
AGCGCAAGGACATGTTGGCTCGTACCCTTGATGGCTTGCTTGATCTGTTCAATGAACGGGTGTTGCCGTTCGATACGGAC
GCGGCACGCCACTATGCTGAACTGGCAGTGAAAGCGAGAAACAATGGGCGCGGCTTTCCCACACCGGATGGCTATATCGC
GGCAATTGCTGCATCACGAGGTTACATAGTAGCTTCCCGCGACACCTCAGCCTATGAGTCAAGTGGTGTGCAAGTCATCA
ATCCGTGGCAATACAGCAAACAAACATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT188617 WP_001110447.1 NZ_CP068825:c1867871-1867578 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT188617 NZ_CP090513:c2454771-2454517 [Xylella fastidiosa subsp. multiplex]
ATGGCCATGTTGACAGTGCGTAATTTGCCTGATGAAGTCCATCGCGCCTTGCGGGTTCGAGCCGCCACACACGGCCGCAG
TACAGAAGCGGAAGTACGTGAGATTTTAACGAACATAGTCAAGCCGGATGAGCGTGTTCGCATTGGCGATGCCTTGGCTA
TCCTGGGACGCGAGATCGGACTAAATAACGAGGATTTCACGACCTTTGATAAGGTGCGAGACAAGACGCAGGCCGAGCCG
ATGAGATTTGAATGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6