Detailed information of TA system TA188616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1619840..1620545
• Replicon: chromosome
• Accession: NZ_CP068825
• Organism: Escherichia coli strain RIVM_C030256

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: JNN79_RS07635
• Protein ID: WP_000539521.1
• Coordinates: 1620159..1620545 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JNN79_RS07630
• Protein ID: WP_001280945.1
• Coordinates: 1619840..1620169 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN79_RS07615	1614997..1615908	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
JNN79_RS07620	1616086..1618434	+	2349	Protein_1477	EAL domain-containing protein	-
JNN79_RS07625	1618442..1619770	+	1329	WP_000086879.1	GGDEF domain-containing protein	-
JNN79_RS07630	1619840..1620169	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
JNN79_RS07635	1620159..1620545	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JNN79_RS07640	1620771..1622096	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
JNN79_RS07645	1622309..1622692	+	384	WP_000497137.1	biofilm formation regulator BssR	-
JNN79_RS07650	1622803..1623918	+	1116	WP_000555038.1	aldose sugar dehydrogenase YliI	-
JNN79_RS07655	1623915..1624541	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T188616 WP_000539521.1 NZ_CP068825:c1620545-1620159 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T188616 NZ_CP090513:1630994-1631281 [Xylella fastidiosa subsp. multiplex]
ATGGCTAAGTCCTATCGACTGACACCGCTGGCCGAGGCCGATCTTGAAGAAATCTGGTTCTACACATTCAGGCACTGGTC
GATAGAGCAGGCCGACAGCTACCACCGCAGCCTAGTTGCAGTTTTCGAGGGGCTGGCAGCAGGTACAAAGCAAGGCTGCC
CTTCTGTTTTGCCGGACTTCAATAAATACCTATGTGGTTCGCATGTAGTGTATTTCATGGATTACGCCGATCACTTGGAT
ATTATCCGCATCCTGCATCAGCGGCAGGATACCGAGCGGTACCTATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT188616 WP_001280945.1 NZ_CP068825:c1620169-1619840 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT188616 NZ_CP090513:1630759-1631001 [Xylella fastidiosa subsp. multiplex]
ATGAGTCGAAACACATCCGTTGCCCTCGGGCCGCACTTCACCAGCTTTATTGATGCCCAAGTCCAGGGCGGTCGCTACGG
TACCGCCAGCGATGTTGTCAGGGCCGGTCTGCGGCTACTTGAGGAACACGAAACAAAGGTGAAGGCACTGCAAGATGCGC
TGAATGTTGGCCACCAATCCGGCGAGCCGCGCCCGTTCGACAGCGAGGTGTTTTTGAGCCGGATGCACACGCAGCATGGC
TAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6