Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1166987..1167605 Replicon chromosome
Accession NZ_CP068825
Organism Escherichia coli strain RIVM_C030256

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JNN79_RS05480 Protein ID WP_001291435.1
Coordinates 1166987..1167205 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JNN79_RS05485 Protein ID WP_000344800.1
Coordinates 1167231..1167605 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN79_RS05445 1162276..1162848 + 573 WP_000779826.1 lipoprotein -
JNN79_RS05450 1162879..1163190 - 312 WP_000409911.1 MGMT family protein -
JNN79_RS05460 1163569..1163922 + 354 WP_000878140.1 DUF1428 domain-containing protein -
JNN79_RS05465 1163964..1165514 - 1551 Protein_1063 cyclic-guanylate-specific phosphodiesterase PdeB -
JNN79_RS05470 1165678..1166148 - 471 WP_000136192.1 YlaC family protein -
JNN79_RS05475 1166264..1166815 - 552 WP_000102559.1 maltose O-acetyltransferase -
JNN79_RS05480 1166987..1167205 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JNN79_RS05485 1167231..1167605 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JNN79_RS05490 1168151..1171300 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
JNN79_RS05495 1171323..1172516 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T188615 WP_001291435.1 NZ_CP068825:c1167205-1166987 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T188615 NZ_CP090513:1359504-1359770 [Xylella fastidiosa subsp. multiplex]
TTGGCCTGGACGATTGATTACACCGACACGGCCAAGCAGCAGTTACGCAAGCTCGACAAGCACATGGCACGGCGCATTGT
CGATTTCATGGATGAGCGCATCGCCGGACTAGAAAATCCACGCAGCAGTGGTAAAGCATTAACCGGACCACTTGGTGGCT
TTTGGCGTTATCGTGTTGGTGATTTTCGGGTCGTCTGCGACATTCAAGATAGCGTTTTGCGCGTCCTTGTAGTGCGTGTT
GGTCACCGGGGCGAGATTTACCGATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT188615 WP_000344800.1 NZ_CP068825:c1167605-1167231 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT188615 NZ_CP090513:1359293-1359520 [Xylella fastidiosa subsp. multiplex]
ATGGCGACTTCGATTCGCCTCTCTCCTGAAATGGAGCAAAGGCTTAACTCTCTTGCCTCCCATACGGGACGCACAAAGGC
TTACTACCTGCGTGAAATCATTGAGCATGGCATTGAGGAAATGGAGGATTACTACCTTGCTGCCGATGTGTTGGAGCGTG
TCCGTCATGGACAAGAACAGGTGCATTCTGCTGCCGACGTGAGGAAAACGCTTGGCCTGGACGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References