188606

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 276085..276582 Replicon chromosome
Accession NZ_CP068825
Organism Escherichia coli strain RIVM_C030256

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JNN79_RS01265 Protein ID WP_001173343.1
Coordinates 276409..276582 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JNN79_RS01260 Protein ID WP_000398619.1
Coordinates 276085..276381 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN79_RS01240 272814..273533 - 720 WP_000611288.1 two-component system response regulator DcuR -
JNN79_RS01245 273530..275161 - 1632 WP_001216477.1 sensor histidine kinase -
JNN79_RS01250 275342..275572 + 231 WP_000373291.1 (4Fe-4S)-binding protein -
JNN79_RS01255 275584..275856 + 273 WP_000405647.1 N-acetyltransferase -
JNN79_RS01260 276085..276381 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JNN79_RS01265 276409..276582 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JNN79_RS01270 276700..278217 - 1518 WP_000003806.1 lysine--tRNA ligase -
JNN79_RS01275 278454..279911 - 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 276085..285435 9350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T188606 WP_001173343.1 NZ_CP068825:276409-276582 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T188606 NZ_CP090512:1550-1876 [Xylella fastidiosa subsp. multiplex]
ATGAAACGCGGCGATGTTTACATGGTCGATCTGGAGCCAACCGCCGGGCACGAACAACGCGGTCACCGTCCGGTGGTTGT
TATCTCCAGTGAGCGATTCAATCGGCTTACCTCATGTCCGGTGATCCTTCCAATCACAAACGGCGGCGAGTTCGCTTCCC
GCCTCGGGTTCGCTGTGGAATTACTAGGTACGATGACAACGGGCATCGTTCGATGTGATCAACCACGTGCACTCGATCTG
CTCGCTCGAAACGCCCGCAAAGTGGAAAGCCTCCCACCTGGCGTCTTGGCCGATGTCTTGGCAAAGGCTGGCACGATCTT
CCAGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT188606 WP_000398619.1 NZ_CP068825:276085-276381 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT188606 NZ_CP090512:1290-1553 [Xylella fastidiosa subsp. multiplex]
ATGCACACCACAAACCTTCGTAAAGTCGGCGGATCGATCATGCTAGCCGTTCCCCCCGCTTTCCTTGATCAGCTTCATCT
TGAGGTCGGCGCAGCGGTTGGCTTAGTTATCACAGACGGCCGCCTGGTCATTGAGCCAGTCCTACATCCTCAGTACACGC
TGGATCAACTGCTGGCCGAAGCGGAAGCTTCCGGGGCTTACCCGTTGCCGCCCGAGGAACGTGAATGGGTCGATGCTCCA
GCAGTAGGACGCGAACTGATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References