188604

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4757387..4757884 Replicon chromosome
Accession NZ_CP068824
Organism Escherichia coli strain RIVM_C030197

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JNN63_RS22945 Protein ID WP_001173343.1
Coordinates 4757387..4757560 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JNN63_RS22950 Protein ID WP_000398619.1
Coordinates 4757588..4757884 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN63_RS22935 4754057..4755514 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
JNN63_RS22940 4755751..4757268 + 1518 WP_001550472.1 lysine--tRNA ligase -
JNN63_RS22945 4757387..4757560 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JNN63_RS22950 4757588..4757884 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JNN63_RS22955 4758113..4758385 - 273 WP_000405647.1 N-acetyltransferase -
JNN63_RS22960 4758397..4758627 - 231 WP_001550470.1 (4Fe-4S)-binding protein -
JNN63_RS22965 4758807..4760438 + 1632 WP_001216477.1 sensor histidine kinase -
JNN63_RS22970 4760435..4761154 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4727954..4757884 29930


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T188604 WP_001173343.1 NZ_CP068824:c4757560-4757387 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T188604 NZ_CP090511:c2492617-2492315 [Xylella fastidiosa subsp. multiplex]
ATGGAGAAAGGCACACCCCACTACAAGCTACCAGTCGTCAAAGCCTTGGTGAAAGCTGGCAAGGTCAGGGCTACAGCGAC
CGCCTACCAAGGAGCACGTGAGCTTGGCATCAAAGATTTAACTGGAATGTGCGCCGTTGTCTTGGCGCTCACGTCTGCCG
ACTTCTACAAGAGTATGACGACCTACACGGATCACACAGTATTCCAGGATGTGTACCACGCCAAGACGGCAAGCGGTGAT
GAGGTGTACCTGAAGTTAACTGTCATTGATGACGTGTTGATTGTTTCCTTCAAGGAGTTATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT188604 WP_000398619.1 NZ_CP068824:c4757884-4757588 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT188604 NZ_CP090511:c2492312-2491911 [Xylella fastidiosa subsp. multiplex]
ATGAGATGCCCATGCTGCGGCGCGGCAGAACTGATCCACGACACGCGCGACATGCTTTACACCTACAAGAGCGAAACTAC
CAGTATCCCAACGGTGACCGGCGACTTCTGCCCGGCTTGTGGCGAAGTCGTCTTGGACCGTGAACATGGTGATCGTTACA
GCGAATTGGTCGGCCTGTTCCAACGTCAGGTGAATTCGGCCTACGTCGATCCTGGTTACATTACCAAGGTACGCCGGAAG
CTTGATCTCGATCAGCGGCAAGCTGCCGAACTGTTCGGTGGTGGTGTTAATGCTTTCTCACGTTACGAGAACGGTAAAAC
CAAGCCGCCGCTGTCTTTGGTTAAGTTATTCAAGCTTCTGGATCGCCACCCCGATTTGCTCAACGAAGTGAAGAGTTTCT
AA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References