Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3828614..3829232 Replicon chromosome
Accession NZ_CP068824
Organism Escherichia coli strain RIVM_C030197

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JNN63_RS18430 Protein ID WP_001291435.1
Coordinates 3829014..3829232 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JNN63_RS18425 Protein ID WP_000344800.1
Coordinates 3828614..3828988 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN63_RS18415 3823703..3824896 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JNN63_RS18420 3824919..3828068 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
JNN63_RS18425 3828614..3828988 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JNN63_RS18430 3829014..3829232 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JNN63_RS18435 3829404..3829955 + 552 WP_000102564.1 maltose O-acetyltransferase -
JNN63_RS18440 3830071..3830541 + 471 WP_000136192.1 YlaC family protein -
JNN63_RS18445 3830705..3832255 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JNN63_RS18450 3832297..3832650 - 354 WP_001550741.1 DUF1428 family protein -
JNN63_RS18460 3833029..3833340 + 312 WP_000409911.1 MGMT family protein -
JNN63_RS18465 3833371..3833943 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T188596 WP_001291435.1 NZ_CP068824:3829014-3829232 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T188596 NZ_CP090511:1419796-1420092 [Xylella fastidiosa subsp. multiplex]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCGCGTCTTGATCGGCTCGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGTGCTGGCATATCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATTCTCTTACTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTGTGGAAGGATTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT188596 WP_000344800.1 NZ_CP068824:3828614-3828988 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT188596 NZ_CP090511:1420096-1420401 [Xylella fastidiosa subsp. multiplex]
ATGAATAATGAGACTTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCTGTCGCTCGTGCTCGTAATTTAA
GTCAACTTGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTATCGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAAGCGCTTGGTTTACGGATGTCATTCACACCTTCCTCTATGAGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References