Detailed information of TA system TA188595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3426073..3426778
• Replicon: chromosome
• Accession: NZ_CP068824
• Organism: Escherichia coli strain RIVM_C030197

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: JNN63_RS16665
• Protein ID: WP_000539521.1
• Coordinates: 3426073..3426459 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JNN63_RS16670
• Protein ID: WP_001280945.1
• Coordinates: 3426449..3426778 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN63_RS16645	3422077..3422703	+	627	WP_001295292.1	glutathione S-transferase GstB	-
JNN63_RS16650	3422700..3423815	-	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
JNN63_RS16655	3423926..3424309	-	384	WP_000497137.1	biofilm formation regulator BssR	-
JNN63_RS16660	3424522..3425847	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
JNN63_RS16665	3426073..3426459	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JNN63_RS16670	3426449..3426778	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
JNN63_RS16675	3426848..3428176	-	1329	WP_001550887.1	GGDEF domain-containing protein	-
JNN63_RS16680	3428184..3430532	-	2349	WP_001550886.1	EAL domain-containing protein	-
JNN63_RS16685	3430710..3431621	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T188595 WP_000539521.1 NZ_CP068824:3426073-3426459 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T188595 NZ_CP090511:c1052574-1052095 [Xylella fastidiosa subsp. multiplex]
ATGCAGATTTCGACACCTCATTCACTCACGGCAGCGCATCGACTTGATGAATTCAATTGCGGTGAGCCATCGCTGGATGA
TTGGCTTAAACGACGCGCACTGACCAACCATTTAAACGGGGCCAGCCGGACCTTCGTGGTTATCGATGCCAACCAATACG
TGCTTGGGTACTACGCTTTGGCAGCCGGGGCTGTAGCGCATCAAGAGGCCACCCGTGCTATCCGACGTAACATGCCTGAT
CCAGTGCCGGTCATGGTGCTCGGAAGGCTGGCTGTGGATACTCGTGCTCAAGGCATCAAACTCGGTGCGGCCTTGCTTAA
AGATACGGTAACTCGTGTGCAGTCCATTGCAGAGAACGCCGGCGTCAGGGCATTGGTAGTCCATGCGTTGAACGAGCGCG
CAAAGCAATTCTACGAGTACTATGGTTTTCGAGCGTCACCAATGCACCTGATGACCTTGATGCTTCCGATTAAATTCTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT188595 WP_001280945.1 NZ_CP068824:3426449-3426778 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT188595 NZ_CP090511:c1052849-1052577 [Xylella fastidiosa subsp. multiplex]
ATGCGCGACGCTTCCATCAACTTACGTGCCATGCCTGAGCAGCGCGACTTGATTGACCAGGCTGCTAATCTATTAGGCAA
GAATCGCTCAGACTTCATGCTAGAAGCTGCGTGCGAGCGTGCGAAAGCAATTATCCTGGATCAAGTCTTTTTCAATCTGG
ACGAAGAGAGGTTCCGGCAATTCACTGTGTTGCTTAATGCGCCACAAGACACCAATCCAGGCCTTAATCGCCTGATGGCA
ATTAAGGCTCCGTGGAGCATCAGCAATACGTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6