Detailed information of TA system TA188593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2702312..2702950
• Replicon: chromosome
• Accession: NZ_CP068824
• Organism: Escherichia coli strain RIVM_C030197

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: JNN63_RS12975
• Protein ID: WP_000813795.1
• Coordinates: 2702774..2702950 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JNN63_RS12970
• Protein ID: WP_076797675.1
• Coordinates: 2702312..2702728 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN63_RS12950	2697464..2698405	-	942	WP_001251315.1	ABC transporter permease	-
JNN63_RS12955	2698406..2699419	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
JNN63_RS12960	2699437..2700582	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
JNN63_RS12965	2700827..2702233	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
JNN63_RS12970	2702312..2702728	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JNN63_RS12975	2702774..2702950	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JNN63_RS12980	2703172..2703402	+	231	WP_023910283.1	YncJ family protein	-
JNN63_RS12985	2703494..2705455	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JNN63_RS12990	2705528..2706064	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
JNN63_RS12995	2706117..2707328	+	1212	WP_071591517.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T188593 WP_000813795.1 NZ_CP068824:c2702950-2702774 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T188593 NZ_CP090511:c477005-476751 [Xylella fastidiosa subsp. multiplex]
ATGAAGCGTAAGCACGCTAAAACTCTCAGCGCTATATACACACGCCCTGTTTCGGCCAACATTCAATGGCGCGATATTGA
AGCGCTGTTCGTGGAGCTTGGAGCGAAAGTAGAAGAACGTGAAGGTTCTCGTATGTTGGTGCGGCTGTTCGGTGAACGTC
GGATATTCCACCGGCCCCACCCTTCGCCTAACACTGACAAGGGTGCTGTTGAATCAATCCGAGCTTGGTTGAAATCGAAC
GGAGTTACACCATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT188593 WP_076797675.1 NZ_CP068824:c2702728-2702312 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT188593 NZ_CP090511:c476754-476419 [Xylella fastidiosa subsp. multiplex]
ATGAAGAACATCATGACTATTGAGAGCTTTAAAGCCATCATTACCTATGATCCGGAGATCGACATGTTTCGCGGTGAATT
TGTGGGTATCAATGGTGGTGCTGATTTCTATGCAAAAGACCTTAAGGGTCTTCGTCGAGAAGGAGCTATTTCTCTGAAGG
TATTTTTGGAAGCCTGCAAAGAAGACAGCGTGGAACCACGTAAATGCTACTCAGGGAAGTTCAATGCAAGGATTTCTCCT
GAACTGCATGCTTTGGCTTCTGAAGCCGCAGCAGCACAGGGCATCAGTTTGAACCAATTTGTAGAACAAGCGATTCAGCA
TGAAGTCCACACCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1