Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4719273..4719889 Replicon chromosome
Accession NZ_CP068823
Organism Escherichia coli strain RIVM_C029494

Toxin (Protein)


Gene name vapC Uniprot ID A0A176ZIX8
Locus tag JNN66_RS22325 Protein ID WP_001129490.1
Coordinates 4719273..4719647 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1M0CY48
Locus tag JNN66_RS22330 Protein ID WP_001275523.1
Coordinates 4719647..4719889 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN66_RS22310 4716776..4717678 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
JNN66_RS22315 4717675..4718310 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
JNN66_RS22320 4718307..4719236 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
JNN66_RS22325 4719273..4719647 - 375 WP_001129490.1 type II toxin-antitoxin system VapC family toxin Toxin
JNN66_RS22330 4719647..4719889 - 243 WP_001275523.1 ribbon-helix-helix domain-containing protein Antitoxin
JNN66_RS22335 4720109..4720327 - 219 WP_001251290.1 ribbon-helix-helix domain-containing protein -
JNN66_RS22340 4721001..4722815 - 1815 WP_000226332.1 hypothetical protein -
JNN66_RS22345 4723231..4724172 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
JNN66_RS22350 4724217..4724654 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-48)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.19 Da        Isoelectric Point: 7.0126

>T188576 WP_001129490.1 NZ_CP068823:c4719647-4719273 [Escherichia coli]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Download         Length: 375 bp

>T188576 NZ_CP090508:c1156641-1156237 [Xylella fastidiosa subsp. morus]
GTGAGCGGCTATATGCTCGACACGAATATCATCAGCGATATCATTCGTAACCCATTCGGGGCGGTTGCTTGCCGTATTGA
ACACGTTGGGTATCCGAACATTTGCACGAGCGTTATCGTGGCGGCTGAACTTCGCTATGGATGCACGAAAAATGGCTCGG
TAAAGCTGCTGAGCAGGGTACAGGACATTCTTAAAACGCTGCCGCTCCTGCCTTTGGAAATACCTGTTGACACAACATAT
GGCAGTATCCGTGCTGAGCTGGAGGCTGCGGGTCAGCTGATCGGCGCTAATGATCTATTGATTGCTGCGCATGCTTACGT
ACTCGGGCTGACTTTGGTCACGGACAACACCCGCGAGTTCAGCCGAATTCGCGGCTTGGATGTGCAGAACTGGCTAGAGA
GGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9290.54 Da        Isoelectric Point: 4.3774

>AT188576 WP_001275523.1 NZ_CP068823:c4719889-4719647 [Escherichia coli]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSAFGIWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT188576 NZ_CP090508:c1156925-1156638 [Xylella fastidiosa subsp. morus]
ATGGATGCCGTGCCGATGCCAATTTCATCTCCGTTATCACGTACCAAGGAAGCGAAGCTATTCCGAAATAATCGTAGCCA
GGCGGTACGAATTCCCGTTGAATTCGAGCTGCCGGGCGATCGTGTTCTGATCCACCGTGAGGGGAGCAAGCTCATTATTG
AGCCGATCACCAGGCCAACCAATATTGTTGAATTACTGCACGAGTGGCGAAGAGAAACACCACTCGACCCAGAAGATCAA
TTCCCTGATATTGATGACATGCCCGTTCAGCCAGAGGATATTCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A176ZIX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CY48

References