Detailed information of TA system TA188574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4293412..4294007
• Replicon: chromosome
• Accession: NZ_CP068823
• Organism: Escherichia coli strain RIVM_C029494

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: JNN66_RS20320
• Protein ID: WP_000239581.1
• Coordinates: 4293412..4293762 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: JNN66_RS20325
• Protein ID: WP_001223213.1
• Coordinates: 4293756..4294007 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN66_RS20300	4288866..4289888	-	1023	WP_001383778.1	ABC transporter permease	-
JNN66_RS20305	4289902..4291404	-	1503	WP_001095665.1	sugar ABC transporter ATP-binding protein	-
JNN66_RS20310	4291537..4292493	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
JNN66_RS20315	4292803..4293333	+	531	WP_000055075.1	inorganic diphosphatase	-
JNN66_RS20320	4293412..4293762	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
JNN66_RS20325	4293756..4294007	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
JNN66_RS20330	4294219..4294560	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
JNN66_RS20335	4294563..4298342	-	3780	WP_075861634.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T188574 WP_000239581.1 NZ_CP068823:c4293762-4293412 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T188574 NZ_CP090508:c534950-534654 [Xylella fastidiosa subsp. morus]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCACGTCTTGATCGGCTCGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGGGCTGGCATATCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATCCTCTTACTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTATGGAAGGATTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT188574 WP_001223213.1 NZ_CP068823:c4294007-4293756 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT188574 NZ_CP090508:c534650-534345 [Xylella fastidiosa subsp. morus]
ATGAATAATGAGACTTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCTGTCGCTCGTGCTCGTAATTTAA
GTCAACTTGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTATCGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAAGCGCTTGGTTTACGGATGTCATTCACACCTTCCTCTATGAGTTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7