Detailed information of TA system TA188572

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4181858..4182693
• Replicon: chromosome
• Accession: NZ_CP068823
• Organism: Escherichia coli strain RIVM_C029494

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: V0Z9U3
• Locus tag: JNN66_RS19790
• Protein ID: WP_000854750.1
• Coordinates: 4181858..4182235 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2J7L0S7
• Locus tag: JNN66_RS19795
• Protein ID: WP_001278231.1
• Coordinates: 4182325..4182693 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN66_RS19765	4179101..4179478	-	378	WP_000784767.1	hypothetical protein	-
JNN66_RS19770	4179547..4180326	-	780	WP_001088803.1	HNH endonuclease	-
JNN66_RS19775	4180933..4181079	-	147	WP_001290178.1	hypothetical protein	-
JNN66_RS19780	4181164..4181361	-	198	WP_000839280.1	DUF957 domain-containing protein	-
JNN66_RS19785	4181373..4181861	-	489	WP_000761685.1	hypothetical protein	-
JNN66_RS19790	4181858..4182235	-	378	WP_000854750.1	TA system toxin CbtA family protein	Toxin
JNN66_RS19795	4182325..4182693	-	369	WP_001278231.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN66_RS19800	4182856..4183025	-	170	Protein_3874	DUF987 family protein	-
JNN66_RS19805	4183112..4183588	-	477	WP_001367707.1	RadC family protein	-
JNN66_RS19810	4183604..4184089	-	486	WP_000206658.1	antirestriction protein	-
JNN66_RS19815	4184181..4184999	-	819	WP_202852119.1	DUF945 domain-containing protein	-
JNN66_RS19820	4185098..4185331	-	234	WP_001278287.1	DUF905 family protein	-
JNN66_RS19825	4185337..4186014	-	678	WP_001097305.1	hypothetical protein	-
JNN66_RS19830	4186162..4186842	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	tet(B)	fimB	4155890..4244267	88377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13919.90 Da
• Isoelectric Point: 8.2905

>T188572 WP_000854750.1 NZ_CP068823:c4182235-4181858 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T188572 NZ_CP090508:c531345-531046 [Xylella fastidiosa subsp. morus]
ATGACCTACACGGTAAAACGACTTGAAGGGTTCTCTGACTGGCTAAAGGGCCTAAAGGATGGGCTGGCAAGGCAGCGCCT
CATCAAGCGCTTACGCAAGGTGCAGCTCGGCAACTTTGGAGACGTGCAGCCAGTGGGTGAAGGCGTGTTTGAAATGCGCG
AACACTTCGGACCTGGCTGGCGTATGTACTACGTGCAGCGAGGCAGCTTTCTCATCGTGATGCTCGGCGGCGGCGACAAG
TCAACGCAGCAATCAGACATTCGTCGGGCCATTGAGCTAGCAAAATCTTTAGAGGATTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT188572 WP_001278231.1 NZ_CP068823:c4182693-4182325 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLEIMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT188572 NZ_CP090508:c531042-530734 [Xylella fastidiosa subsp. morus]
GTGACCATCACAAAGAAAATCAATGTCTCCGAACTGCCAGAGTTCGATGCCGCCGAATACCTGAACAGCGAAGAGGAGGT
AGCCGCGTATCTCACGGCTGTTCTTGAAGAAAACGATCCAGCACTGTTAGCGGCTGCGCTGGGTGACATTGCCCGTTCAC
GTGGTATGTCTCAGATTGCGAAGGATTCTGGCATCACGCGGGAAGCTCTATACAAGGCTCTTCGGCCTGGCAGTGAACCA
CGCTTCGATACGATAAGCCGCGTCTGTACCGCCCTAGGTATACGTCTAGTAGCGCAACCAATGCATTGA

Structures

Toxin

• AlphaFold DB: V0Z9U3

Antitoxin

• AlphaFold DB: A0A2J7L0S7