Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4122098..4122674 Replicon chromosome
Accession NZ_CP068823
Organism Escherichia coli strain RIVM_C029494

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag JNN66_RS19525 Protein ID WP_001131965.1
Coordinates 4122387..4122674 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag JNN66_RS19520 Protein ID WP_000063158.1
Coordinates 4122098..4122400 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN66_RS19500 4117206..4118426 + 1221 WP_000873756.1 amino acid deaminase -
JNN66_RS19505 4118694..4120844 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
JNN66_RS19510 4120894..4121097 + 204 WP_000467859.1 YbdD/YjiX family protein -
JNN66_RS19515 4121108..4122064 + 957 WP_001361361.1 GTPase -
JNN66_RS19520 4122098..4122400 - 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
JNN66_RS19525 4122387..4122674 - 288 WP_001131965.1 BrnT family toxin Toxin
JNN66_RS19530 4122871..4124037 + 1167 WP_000800823.1 restriction endonuclease -
JNN66_RS19535 4124082..4124255 + 174 Protein_3821 SAM-dependent DNA methyltransferase -
JNN66_RS19540 4124249..4124917 + 669 Protein_3822 DUF3387 domain-containing protein -
JNN66_RS19545 4125132..4125470 + 339 WP_000132633.1 endoribonuclease SymE -
JNN66_RS19550 4125512..4125676 - 165 WP_000394263.1 DUF1127 domain-containing protein -
JNN66_RS19555 4125853..4127265 + 1413 WP_000199309.1 PLP-dependent aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T188571 WP_001131965.1 NZ_CP068823:c4122674-4122387 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T188571 NZ_CP090508:c531042-530734 [Xylella fastidiosa subsp. morus]
GTGACCATCACAAAGAAAATCAATGTCTCCGAACTGCCAGAGTTCGATGCCGCCGAATACCTGAACAGCGAAGAGGAGGT
AGCCGCGTATCTCACGGCTGTTCTTGAAGAAAACGATCCAGCACTGTTAGCGGCTGCGCTGGGTGACATTGCCCGTTCAC
GTGGTATGTCTCAGATTGCGAAGGATTCTGGCATCACGCGGGAAGCTCTATACAAGGCTCTTCGGCCTGGCAGTGAACCA
CGCTTCGATACGATAAGCCGCGTCTGTACCGCCCTAGGTATACGTCTAGTAGCGCAACCAATGCATTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT188571 WP_000063158.1 NZ_CP068823:c4122400-4122098 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT188571 NZ_CP090508:c531345-531046 [Xylella fastidiosa subsp. morus]
ATGACCTACACGGTAAAACGACTTGAAGGGTTCTCTGACTGGCTAAAGGGCCTAAAGGATGGGCTGGCAAGGCAGCGCCT
CATCAAGCGCTTACGCAAGGTGCAGCTCGGCAACTTTGGAGACGTGCAGCCAGTGGGTGAAGGCGTGTTTGAAATGCGCG
AACACTTCGGACCTGGCTGGCGTATGTACTACGTGCAGCGAGGCAGCTTTCTCATCGTGATGCTCGGCGGCGGCGACAAG
TCAACGCAGCAATCAGACATTCGTCGGGCCATTGAGCTAGCAAAATCTTTAGAGGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References