188568

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3624475..3625312 Replicon chromosome
Accession NZ_CP068823
Organism Escherichia coli strain RIVM_C029494

Toxin (Protein)


Gene name itaT Uniprot ID A0A1M0D5U3
Locus tag JNN66_RS17150 Protein ID WP_000227782.1
Coordinates 3624770..3625312 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID B1LJI1
Locus tag JNN66_RS17145 Protein ID WP_001353405.1
Coordinates 3624475..3624786 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN66_RS17120 3619495..3620442 + 948 WP_001239437.1 cytochrome o ubiquinol oxidase subunit II -
JNN66_RS17125 3620464..3622455 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
JNN66_RS17130 3622445..3623059 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
JNN66_RS17135 3623059..3623388 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
JNN66_RS17140 3623400..3624290 + 891 WP_000971327.1 heme o synthase -
JNN66_RS17145 3624475..3624786 + 312 WP_001353405.1 DUF1778 domain-containing protein Antitoxin
JNN66_RS17150 3624770..3625312 + 543 WP_000227782.1 GNAT family N-acetyltransferase Toxin
JNN66_RS17155 3625368..3626212 - 845 Protein_3354 sel1 repeat family protein -
JNN66_RS17160 3626619..3627983 + 1365 WP_001000978.1 MFS transporter -
JNN66_RS17165 3628111..3628602 - 492 WP_001138904.1 nucleotide binding protein YajQ -
JNN66_RS17170 3628770..3629681 + 912 WP_000705836.1 2-dehydropantoate 2-reductase -
JNN66_RS17175 3629644..3630234 + 591 WP_001276309.1 protein deglycase YajL -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19957.31 Da        Isoelectric Point: 9.1763

>T188568 WP_000227782.1 NZ_CP068823:3624770-3625312 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDIIYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCNFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T188568 NZ_CP090508:c483696-483403 [Xylella fastidiosa subsp. morus]
ATGACCGAGAAACTGACGAGCTACGATCCAGCCGAGGACTTGACCACTGACCAGGCCATCGCCGATTTCATGGCAGCTGC
GTTCGGGACGAACGATCCTGCCTACGTTGCCCACGCGCTGGGCGTCGTTGCCCGCGCCAAGGGCATGACGCAGATCGCCA
GCCAGACAGGGCTATCGCGCGAACAGCTCTACCGCTCGTTCAGTGCCGAGGGCAACCCGACGCTGCGCACAACATTGGCC
GTGATGAAGGCGCTCGGGATCGAGCTGTCTGCGAAACCGTCTGGTGTTCACTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11370.09 Da        Isoelectric Point: 10.4825

>AT188568 WP_001353405.1 NZ_CP068823:3624475-3624786 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT188568 NZ_CP090508:c483995-483699 [Xylella fastidiosa subsp. morus]
ATGATTGAATTGAAGCAGACTGACACCTTCCGCAAGTGGCGGGAGAAACTCAAGGATGCGCGCGCCCGCTCAGCCATCGC
CTCGCGCCTCGACCGCTTGGCGTTCGGCCATGTCGGCGACGCCGAGCCAGTAGGGAAAGGTGTCAGCGAGCTTCGCATCA
ACTACGGCCCCGGTTACCGGGTGTATTTCCAGCGGCGTGGCGACACGATCTACTTGCTGCTTTGCGGCGGTGACAAAGGA
TCACAAGCGCGCGACATCAAGACTGCGCTGCACCTGTCTGAACAATGGAGCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0D5U3

Antitoxin

Source ID Structure
AlphaFold DB A0A829G7G3

References