Detailed information of TA system TA188565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3212597..3213302
• Replicon: chromosome
• Accession: NZ_CP068823
• Organism: Escherichia coli strain RIVM_C029494

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1M2J6W7
• Locus tag: JNN66_RS15185
• Protein ID: WP_000539524.1
• Coordinates: 3212597..3212983 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JNN66_RS15190
• Protein ID: WP_001280945.1
• Coordinates: 3212973..3213302 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN66_RS15165	3208601..3209227	+	627	WP_001295292.1	glutathione S-transferase GstB	-
JNN66_RS15170	3209224..3210339	-	1116	WP_075861885.1	aldose sugar dehydrogenase YliI	-
JNN66_RS15175	3210450..3210833	-	384	WP_000497137.1	biofilm formation regulator BssR	-
JNN66_RS15180	3211046..3212371	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
JNN66_RS15185	3212597..3212983	+	387	WP_000539524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JNN66_RS15190	3212973..3213302	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
JNN66_RS15195	3213372..3214700	-	1329	WP_000086870.1	GGDEF domain-containing protein	-
JNN66_RS15200	3214708..3217056	-	2349	WP_187809982.1	EAL domain-containing protein	-
JNN66_RS15205	3217233..3218144	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14321.46 Da
• Isoelectric Point: 9.9521

>T188565 WP_000539524.1 NZ_CP068823:3212597-3212983 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSRSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T188565 NZ_CP090507:c18787-18518 [Xylella fastidiosa subsp. morus]
ATGGCATGGAAGGTTGAGCTTTCGTCGTTGGCTCAAAAAAACCTTGATCAGCTTGACCCACAAATTGCCCTGCGTGTCCT
ATCATTTCTCCATGAGCGTGTGGCTCCTTTGGACGATCCGCGCAGCATCGGTGAATCCCTCAAGGGTTCGCGCCTTGGTG
AGTTTTGGAAGTACCGCGTGGGTGATTACCGGATTATCAGCAGCATTGAGGACGGCGCTTTGCGCATCCTCGTTGTGAAG
ATTGGCAACCGCCGCGAGGTTTACCGCTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT188565 WP_001280945.1 NZ_CP068823:3212973-3213302 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT188565 NZ_CP090507:c19001-18771 [Xylella fastidiosa subsp. morus]
ATGGCCTCCGTATCACTGCGTTTGCCTGATGAAGTTACACAACGACTTGAGAACTTAGCAGCGCTCACAGGGCGTAGCAA
AACCTTCTACATGGTGGAAGCCATCCGCGAACACATCGACGACCTAGAAGACTTGTACCTAGCCGAGCGTGAGCTTGAAG
CGATCCGTTCTGGCAATTCGCAAACCGTACCACTCGAAGAAGTGATGCAACGCTATGGCATGGAAGGTTGA

Structures

Toxin

• AlphaFold DB: A0A1M2J6W7